Chloroplasts

Question 1

Stromules

Answer 1

Connect chloroplasts

Long, stroma filled tubules that are highly dynamic (can rapidly extend and contract)

Allows for metabolite transfer, communication between chloroplasts and other organelles

Question 2

FtsZ

Answer 2

Filmanetous temperature-sensitive Z1/2

Soluble stromal proteins
Homologs of the bacteria division protein FtsZ

Question 3

ARC6

Answer 3

Accumulation and Replication of Chloroplasts 6

Integral inner (envelope) membrane spanning proteins localized at the equator in a dividing chloroplast (stabilizes 
 and controls tightening of FtsZ ring)

Question 4

PDV1/2

Answer 4

Plastid Division 1 and 2

Hetero-dimeric, integral outer transmembrane proteins recruited (via binding to ARC6) to the midway point in a dividing chloroplast

IMS facing domains of PVD1/2 interact with IMS facing domain of ARC6

Also binds to ARC5

Question 5

ARC 5

Answer 5

Accumulation and Replication of Chloroplasts 5

Cytoplasmic facing domains of PDV1/2 bind to ARC5 at the site of division on the chloroplast outer membrane surface

ARC5 is a cytoplasmic, soluble dynamin-related GTPase protein

Question 6

Transit Peptide

Answer 6

N terminal targeting signal on stromal proteins

Recognized by TOC

Question 7

TOC Complex

Answer 7

Translocase of the Outer Chloroplast membrane

Multi protein complex
Transit peptide binds to the receptor protein of the TOC complex (Toc34) in a GTP dependent manner

Question 8

TIC

Answer 8

Translocase of the Inner Chloroplast membrane

Multi protein complex

Question 9

Stromal Processing Enzyme

Answer 9

Cleaves transit peptide

Question 10

Thylakoid Transfer Domain

Answer 10

Exposed once transit peptide is cleaved

Allows for import into thylakoid