ER to Golgi Transport

Question 1

COPs

Answer 1

Coat Proteins

Recognize and concentrate specific protein/lipid components that will be incorporated into the budding vesicle

Mediate ERES membrane curvature and formation of the budding vesicle

COP I,II, Clathrin

Question 2

COP II

Answer 2

Anterograde transport to Golgi

Question 3

COP I

Answer 3

Retrograde transport back to ERES

Question 4

Clathrin

Answer 4

Transport from Golgi to endosomes or from PM to endosomes

Question 5

CGN

Answer 5

cis Golgi Network

Consists of an interconnected network of vesicles and tubules located on the cis face (side) of the Golgi complex

Often adjacent to EREs

Question 6

Rab Proteins

Answer 6

Large family of lipid anchored, GTP binding membrane proteins

Located on all transport vesicles and acceptor membranes

Question 7

Tethering Factors/ Rab Effectors

Answer 7

Highly elongated (fiber like, rod shaped) proteins or

Components of large, multi-protein complexes

Question 8

SNARE Proteins

Answer 8

(Soluble NSF Attachment protein REceptors)

Large family of integral membrane bound proteins located on all transport vesicles and all acceptor membranes

Question 9

SNARE motif

Answer 9

Present in all SNARE proteins

Cytoplasmic facing domain that extends from the membrane surface

Involved in SNARE-SNARE protein binding

Question 10

KDEL Receptor

Answer 10

Integral transmembrane protein

Lumenal facing domain binds to KDEL sequence of escaped protein

Cytoplasmic facing domain of KDEL binds to COPI components

Question 11

Dilysine Sequence

Answer 11

On C terminus

KKxx