Media Components Flashcards
What are the seven components of media
- macro and micronutrients
- vitamins
- amino acids or other nitrogen supplements
- sugars
- undefined organic supplements
- solidifying agents
- growth regulators
what are defined components
defined overtime by analysis of plant tissue composition and trial and error
6 undefined organic components
- coconut liquid endosperm
- yeast extract (B vitamins)
- Malt extract
- tomato juice
- casein hydrolysate (amino acid mixture)
- activated charcoal
What are 2 hormones used?
- auxins (cell differentiation, enlargement)
- cytokinin (cell division)
a high ratio of cytokinin to auxin produces?
shoots
- the reverse produces roots
a high ratio of auxin to cytokinin determines what?
tissue differentiation
What are some auxins used/ their purpose
IAA, IBA, 2,4-D, NAA
- stimulate callus production and cell growth
- initiate roots
- induce somatic embryogenesis
- stimulate growth from shoot tip cultures
What are some cytokinins used/ their purpose
BAP, BA, kinetin, zeatin
- stimulate cell division
- induce shoot formation
- induce axillary shoot proliferation
- inhibit root formation
what are some antibiotics used?
- ampicillin
- kanamycin/antamycin
- carbenicillin, cefotaxime, tetracycline
media prep considerations
- solid media ph 5.7-5.8
- autoclave effects sugars, agars, antibiotics, and hormones
- can used filtration
what are the 6 macronutrients and their role
N- nucleic acids, proteins, chlorophyll
P- ATP, DNA
K- cell division
Mg- central element in chlorophyll
Ca- cell walls (pectin)
S- proteins
