Lab 3 - microspore culture Flashcards
1
Q
which stage of development is optimal for accession?
A
late uninucleate stage of pollen development (embryonic at this stage)
2
Q
donor plant requirements
A
- healthy plants
- bud cleanliness
- closed buds
- donor plant temp below 20C
- free of pests
- plants fertilized regularly
3
Q
role of sucrose
A
- to prevent osmotic shock of the cells
4
Q
why concentration of microspores is critical
A
- 75,000 to 100,000 microspores/mL
- too few: to little cell contact
- too many: toxin production
5
Q
double haploid principles
A
- useful breeding tool (rapidly produce homozygous lines)
- fixed genes (phenotype=genotype)
- screenable at early stages (genetic and phenotypic screening, PCR marker assisted breeding)
6
Q
how does colchicine work?
A
- plantlets can be induced to be diploid with colchicine as it inhibits mitotic spindle formation
