MBT

Question 1

Describe the advantages/disadvantages of introducing genes via non-integrating plasmids over retroviral transduction

Answer 1

A1: Does not cause insertional mutagenesis&raquo_space; cancer

A2: Lower risk of stimulating immune response
+ lower risk of pathogenicity

D1: No integration&raquo_space; expression of transgenes is short-lived

D2: Lower efficiency of transferring transgenes

Question 2

Why is the individual phenotypically normal but has RFLP 2, which normally indicates BRCA-1 mutation (dominant)?

Answer 2

1. Individual is still young and has not developed cancer
2. Crossing over b/w RFLP locus and BRCA-1 locus
   » RFLP 2 linked to normal BRCA-1

Question 3

Compare SCR with PCR

Answer 3

1. Nature of primers
2. Location - nucleus vs. thermocycler
3. Enzyme
4. Proofreading
5. Unzipping of template DNA - helicase vs. high temp.
6. Synthesis of primers by primase