mass spectrometer Flashcards
use
determine all the isotopes present in a sample of an element
RAM
(isotopic mass x %abundance)/ 100%
what is the molecule in the mass spectrometer that is equal to the Mr
the peak with the largest m/z (molecular ion)
steps of a mass spectrometer
ionisation
acceleration
deflection
detection
ionisation
a high energy beam of electrons is used to knock an electron out of an atom making the neutral atom ionised to be a positive ion.
accleration
Positively charged ions are accelerated using a magnetic field.
deflection
charged ions are deflected using an electromagnetic field
- heavier ions will take the stronger field strength to be deflected
detection
ions hit a negatively charged detector plate where a signal is generated.
- the greater the signal, the more ions are hitting the plate