MANUAL: PART 3: Medium and materials

Question 1

It contains suitable environment and nutrients for microbes to grow.

Answer 1

Culture medium

Question 2

A solidifying agent extracted from seaweed composed of complex sugars: liquid at 100° C and solid at 40°C.

Answer 2

Agar

Question 3

A medium to observed morphologies of microorganism. Generally requires 15-20ml of molten agar.

Answer 3

Agar plate

Question 4

A medium that generally requires 7ml of molted agar dispensed into tubes before autoclaving and slanted after sterilization.

Answer 4

Agar slant

Question 5

A medium where no agar or solidifying agent is used.

Answer 5

Broth

Question 6

A subculturing instrument where it is used to transfer small amount of microbes to plates, tubes, and broth.

Answer 6

Inoculating loop

Question 7

A subculturing instrument where it is used to transfer microbes to broth by stabbing into agar deep tube or semisolid media.

Answer 7

Inoculating needle

Question 8

A subculturing instrument, it is used to transfer culture media from one vessel to another.

Answer 8

Pipette

Question 9

A type of pipette where it has etched ring on mouthpiece.

Answer 9

Serological pipette

Question 10

A type of pipette where it has no etched ring.

Answer 10

Mohr pipette

Question 11

An equipment used for destroying all forms of life: set at 15 minutes; 15 psi; and 121°C.

Answer 11

Autoclave

Question 12

An equipment use to maintain optimum temperature for microorganism to grow: typically set at 37°C, for 18-24 hours.

Answer 12

Incubator

Question 13

An equipment use to storage and make microbes bacteriostatic. Typically set at 4°C.

Answer 13

Refrigerator