LAB 6: Define and complex Flashcards
This contains the nutrients to support growth of microorganism that contains C and N that are essential for metabolism.
Culture media
What are the two class of culture media?
Define media
Complex media
What media has the specific organic and inorganic compounds for a specific bacteria?
Define media
What media that is a general that support growth of wide range of microorganism: composed of plant and animal digest.
Complex media
A type of complex media that is mixed with blood, serum, or egg yolk.
Enriched media
This encourages the growth of a low abundance of bacteria from a sample.
Enrichment media
This allows the growth of a selected bacteria, and inhibits the growth of other bacteria.
Selective media
This media distinguish group or specific bacterium through changes of growth or medium.
Differential media
Give the 5 pH indicator:
Bromothymol blue
Methyl red
Natural red
Phenol Red
Eosin Y
Give the pH color of bromothymol blue from Acidic (A) to Alkali(K):
A: Orange or Blue
K: Green
Give the pH color of methyl red from Acidic (A) to Alkali(K):
A: Red or orange
K: Yellow
Give the pH color of phenol red from Acidic (A) to Alkali(K):
A: Red or dark orange
K: Yellow
Give the pH color of Eosin Y from Acidic (A) to Alkali(K):
A: no color
K: Black or metallic green sheen
What type of media is blood agar?
Enriched and differential media
Has tryptic soy agar (TSA)
What bacteria does blood agar cultivates?
Fastidious bacteria: Streptococci and Staphylococci
What does blood agar differentiates?
Ability of streptococci/staphylococci to hemolysin.
Known as partial hemolysin.
alpha hemolysin
Known as complete hemolysin
Beta hemolysin
A cultivated bacteria in blood agar shows growth without hemolysin.
Gamma hemolysin
A test that is selective to staphylococci and bacteria that can tolerate 7.5% salt content.
Mannitol Salt Agar
what is the Growth Inhibitor of MSA?
Due to salt concentration 7.5% that causes hypertonic.
What does Mannitol Salt Agar differentiates?
Bacteria Mannitol fermenters
A test that is selective to gram negative, and differentiates bacteria lactose fermenters.
MacConkey Agar
What is the growth inhibitor of MacConkey Agar?
Bile salt or Crystal Violet.
A test that is selective for gram negative bacteria, and differentiates a lactose and sucrose fermenter.
Eosin Methylene Blue agar
Growth inhibitor of EMBa?
Methylene Blue that prevents gram positive growth.
Difference between metallic green sheen, and black color Eosin in EMBA?
Black color: rapid or lactose fermenter
Green color: Vigorous or rapid lactose fermenter
