LAB 6: Define and complex

Question 1

This contains the nutrients to support growth of microorganism that contains C and N that are essential for metabolism.

Answer 1

Culture media

Question 2

What are the two class of culture media?

Answer 2

Define media
Complex media

Question 3

What media has the specific organic and inorganic compounds for a specific bacteria?

Answer 3

Define media

Question 4

What media that is a general that support growth of wide range of microorganism: composed of plant and animal digest.

Answer 4

Complex media

Question 5

A type of complex media that is mixed with blood, serum, or egg yolk.

Answer 5

Enriched media

Question 6

This encourages the growth of a low abundance of bacteria from a sample.

Answer 6

Enrichment media

Question 7

This allows the growth of a selected bacteria, and inhibits the growth of other bacteria.

Answer 7

Selective media

Question 8

This media distinguish group or specific bacterium through changes of growth or medium.

Answer 8

Differential media

Question 9

Give the 5 pH indicator:

Answer 9

Bromothymol blue
Methyl red
Natural red
Phenol Red
Eosin Y

Question 10

Give the pH color of bromothymol blue from Acidic (A) to Alkali(K):

Answer 10

A: Orange or Blue
K: Green

Question 11

Give the pH color of methyl red from Acidic (A) to Alkali(K):

Answer 11

A: Red or orange
K: Yellow

Question 12

Give the pH color of phenol red from Acidic (A) to Alkali(K):

Answer 12

A: Red or dark orange
K: Yellow

Question 13

Give the pH color of Eosin Y from Acidic (A) to Alkali(K):

Answer 13

A: no color
K: Black or metallic green sheen

Question 14

What type of media is blood agar?

Answer 14

Enriched and differential media
Has tryptic soy agar (TSA)

Question 15

What bacteria does blood agar cultivates?

Answer 15

Fastidious bacteria: Streptococci and Staphylococci

Question 16

What does blood agar differentiates?

Answer 16

Ability of streptococci/staphylococci to hemolysin.

Question 17

Known as partial hemolysin.

Answer 17

alpha hemolysin

Question 18

Known as complete hemolysin

Answer 18

Beta hemolysin

Question 19

A cultivated bacteria in blood agar shows growth without hemolysin.

Answer 19

Gamma hemolysin

Question 20

A test that is selective to staphylococci and bacteria that can tolerate 7.5% salt content.

Answer 20

Mannitol Salt Agar

Question 21

what is the Growth Inhibitor of MSA?

Answer 21

Due to salt concentration 7.5% that causes hypertonic.

Question 22

What does Mannitol Salt Agar differentiates?

Answer 22

Bacteria Mannitol fermenters

Question 23

A test that is selective to gram negative, and differentiates bacteria lactose fermenters.

Answer 23

MacConkey Agar

Question 24

What is the growth inhibitor of MacConkey Agar?

Answer 24

Bile salt or Crystal Violet.

Question 25

A test that is selective for gram negative bacteria, and differentiates a lactose and sucrose fermenter.

Answer 25

Eosin Methylene Blue agar

Question 26

Growth inhibitor of EMBa?

Answer 26

Methylene Blue that prevents gram positive growth.

Question 27

Difference between metallic green sheen, and black color Eosin in EMBA?

Answer 27

Black color: rapid or lactose fermenter

Green color: Vigorous or rapid lactose fermenter