MANUAL 4: PART 1: MICROSCOPY

Question 1

It is a measure of how big an object looks to your eye.

Answer 1

Magnification

Question 2

What are the three concept of microscope.

Answer 2

Magnification
Resolution
Contrast

Question 3

Microscope is both parfocal and parcentral when switching lenses. What does it mean?

Answer 3

Parfocal: remains focus
Parcentral: remains center

Question 4

The measurement of the difference in appearance between 2 object.

Answer 4

Contrast

Question 5

It is defined by the physical characteristic of lens in relation to how condenser lens concentration.

Answer 5

Numerical aperture

Question 6

Relation of condenser lens to numerical aperture?

Answer 6

Directly proportional:
higher lens = higher numerical aperture

Question 7

It is called the distance between the tip of the lens and the stage.

Answer 7

Working distance

Question 8

The area of the slide that can be observed.

Answer 8

Field of view

Question 9

This is observed at thicker specimen creating a 3d like.

Answer 9

Depth of field

Question 10

How to compensate refraction index of light when viewing in high magnification?

Answer 10

Add oil immersion oil and use oil immersion objective lens.