Manipulation Of Dna

Question 1

How is complementary dna made?

Answer 1

1-make rna add oligoprimer
2-add reverse transcriptase, dna sysnthesis starts
3-rna partially digested via rnase
4-add dna pol 1 and ligament to seal gaps

Question 2

Describe the steps and temperatures needed for pcr

Answer 2

Denature- heat to 95- makes it into single strands
Primer sticking- reduce to 45-68 allows primers to stick
Extension- temp at 72 to let taq polymerase synthesise dna