Gene Products

Question 1

What mechanism does tRNA use to bind?

Answer 1

Wobble mechanism

As long as codon codes for same aa the tRNA will bind to it

Question 2

How many nucleotides make up tRNA?

Answer 2

75 to 90

Question 3

What loads tRNA with aa?

Answer 3

Amino acyl tRNA synthetase

Question 4

What are the 3 binding sites on the ribosome that allows tRNA to attach?

Answer 4

E
P
A

Question 5

In the elongation stage of translation what sites have peptide bonds between them?

Answer 5

P and a, ribosome translocation so a site is free again

Question 6

How is hnrna modified before translation ?

Answer 6

Capping
Poly a tail added
Splicing

Question 7

What’s the purpose of actinomycin

Answer 7

Binds dna at initiation, prevents elongation

Question 8

What’s the purpose of rifamycin

Answer 8

Stops synthesis by binding to prokaryotic rna polymerase

Question 9

What’s the purpose of alpha amanitin

Answer 9

Toxin, inhibits rna polymerase 2

Question 10

What’s the purpose of streptomycin

Answer 10

Misreads codons

Question 11

What’s the purpose of erythromycin

Answer 11

Binds to 50s , stops translocation

Question 12

What’s the purpose of cycloheximide

Answer 12

Interferes with translocation step

Question 13

What’s the purpose of tetracycline

Answer 13

Stops Binding of aa to ribosome

Question 14

What’s the purpose of puromycin

Answer 14

Causes premature chain termination