MANIPULATING NUCLEIC ACID

Question 1

extract total mRNA from the specimen and separate it by electrophoresis

Answer 1

northern blot

Question 2

methods for mRNA

Answer 2

northern blot
ribonuclease protection
reverse transcription polymerase chain reaction (RT-PCR)

Question 3

designed to reveal the spatial domains of gene expression in a specimen

Answer 3

in situ hybridization

Question 4

FISH

Answer 4

fluorescence in situ hybridization

Question 5

to evaluate DNA extracts from tissue sample

Answer 5

southern blot

Question 6

provide a means to measure and monitor the expression of thousands of genes at once

Answer 6

microarrays

Question 7

wild type alleles are replaced with mutant ones

Answer 7

gene targeting experiments

Question 8

protein product of the mutant gene is less active than the wild type

Answer 8

loss of function mutation

Question 9

mutant gene interferes with the function of the wild-type form

Answer 9

gain of function mutation

Question 10

plasmids are called

Answer 10

cloning vectors

Question 11

cells are incubated in solution that makes them drink in cloned DNA

Answer 11

transfection

Question 12

high voltage pulse pushes the cloned DNA into cell

Answer 12

electropolation

Question 13

cloned gene in solution is injected into the cell nucleus

Answer 13

microinjection

Question 14

cloned DNA is inserted into mobile regions of DNA that has the ability to integrate themselves into the genome of an organism

Answer 14

retroviral vector

Question 15

interrupts translation of mRNA to protein by introducing single strands of RNA targeted to bind with the mRNA

Answer 15

anti-sense rna