Major Proteins involved in Replication? Flashcards
1
Q
what are DNA polymerases?
A
add nucleotides to a strand growing 5’-3’ copying a DNA template 3’-5’
2
Q
what is primase?
A
synthesizes RNA primers
3
Q
what are helicases?
A
these separate parental DNA strands, unwind the helix
4
Q
what are single stranded binding proteins?
A
prevent single strands of DNA from reassociating
5
Q
what is topoisomerase?
A
these relieve torsional strain on parental duplex caused by unwinding
6
Q
enzymes that remove primers?
A
RNase H
Flap endonucleases
7
Q
what is DNA ligase?
A
joins by forming a phosphodiester bond, two adjacent DNA strands that are bound to the same template
8
Q
what is proliferating cell nuclear antigen (PCNA)?
A
enhances processivity of the DNA polymerase; binds to many proteins present at the replication fork
