Enzymes used in Recombinant DNA------------------------------------------ Flashcards
define Restriction Endonucleases
cut double stranded DNA at specific sequences. Used in cloning and some diagnostic assays (e.g., RFLP).
define DNA polymerase
polymerizes a new DNA molecule that is complementary to an existing template strand. It has many uses in molecular biology.
define heat stable DNA polymerases
a class of DNA polymerases, isolated from thermophilic bacteria, that remain active at high temperatures. Taq DNA polymerase (from Thermus aquaticus) is the most common example. Uses include PCR and cycle sequencing.
define DNA ligases
forms a phosphodiester linkage between the 5’-phosphate and the 3’-hydroxyl of adjacent nucleosides on “nicked” DNA. Used in cloning.
define RNA polymerases
polymerizes an RNA molecule that is complementary to an existing DNA template.
define Reverse Transcriptase
polymerizes a DNA molecule that is complementary to an RNA template. It is used in the production of cDNA and to identify transcriptional start-sites of genes.
polymerizes a DNA molecule that is complementary to an RNA template. It is used in the production of cDNA and to identify transcriptional start-sites of genes.
Reverse Transcriptase
polymerizes an RNA molecule that is complementary to an existing DNA template.
RNA polymerases
forms a phosphodiester linkage between the 5’-phosphate and the 3’-hydroxyl of adjacent nucleosides on “nicked” DNA. Used in cloning.
DNA ligases
a class of DNA polymerases, isolated from thermophilic bacteria, that remain active at high temperatures. Taq DNA polymerase (from Thermus aquaticus) is the most common example. Uses include PCR and cycle sequencing.
heat stable DNA polymerases
polymerizes a new DNA molecule that is complementary to an existing template strand. It has many uses in molecular biology.
DNA polymerase
cut double stranded DNA at specific sequences. Used in cloning and some diagnostic assays (e.g., RFLP).
Restriction Endonucleases
