MAJOR EXAM Flashcards
Which of the following IgG subclass is/are the most efficient in crossing the placenta?
a. IgG1
b. IgG2
c. IgG3
d. IgG4
e. Both A and C
f. Both A and B
IgG1and IgG3 ( A and C)
While performing routine postpartum testing for an Rh immune globulin candidate, a weakly positive antibody screening test was found. Anti-D was identified. This antibody is most likely the result of:
a. Massive fetomaternal hemorrhage occurring at the time of this delivery
b. Antenatal administration of RhIg at 28 weeks’ gestation
c. Contamination of the blood sample with Wharton’s jelly
d. Mother having a positive DAT
Massive fetomaternal hemorrhage occurring at the time of this delivery
Titer of individuals after exposure to RhIg:
a. 1
b. 16
c. 32
d. 34
e. None of the above
34
A phenomenon wherein accurate Rh testing can be difficult if the red cells are heavily coated with IgG anti D antibodies;
a. Partial D
b. Genetic weak D
c. Blocked Rh
d. Position effect
Blocked Rh
AHG is used to detect what type of antibody:
a. warm reacting antibody
b. IgG
c. IgD
d. Both a and b
e. Both b and c
Both a and b
Reagent added to counter check the result of Coombs test:
a. Anti-H
b. Group O IgM coated RBC
c. Group O IgG coated RBCs
d. NOTA
Group O IgG coated RBCs
The following disease may cause a positive reaction to DAT except:
a. Lead poisoning
b. Infectious mononucleosis
c. Infectious hepatitis
d. Chronic granulomatous disease
Chronic granulomatous disease
Direct antihuman globulin test is used to diagnose:
a. HDN
b. HTR
c. Autoimmune hemolytic anemia
d. AOTA
AOTA
- It is the reason why blood group O is used as reagent check cells in Coombs test.
a. Presence of expected antibodies
b. Absence of A and B antigens
c. It has more H antigens
d. None of the above
Absence of A and B antigens
Reaction medium used in Antihuman globulin test which removes water in order to concentrate the antibodies present.
a. Albumin
b. DTT
c. Polyethylene glycol
d. LISS
Polyethylene glycol
To validate the reaction obtained in the antiglobulin test, one can:
a. Use green antiglobulin reagent
b. Add IgG-coated red cells to each test tube
c. Add IgG-coated red cells to each positive reaction
d. Add IgG-coated red cells to each negative reaction
Add IgG-coated red cells to each negative reaction
Type of immunoglobulin which is best in complement activation?
a. IgG
b. IgM
c. IgD
d. IgA
IgM
Which of the following IgG subclass is not capable of complement activation?
a. IgG1
b. IgG2
c. IgG3
d. IgG4
IgG4
The pentameric structure of IgM can be reduced into 5 monomers by the use of this agent:
a. Thiol
b. DTT
c. Albumin
d. PEG
DTT
Demonstration of IgG coated RBCs would be best demonstrated by what test?
a. DAT
b. IAT
c. Elution
d. Treatment of DTT
e. None of the above
DAT
Incubation period wherein most of the clinically significant antibodies are already reacting.
a. 120 minute
b. 10-15 minutes
c. 30 minutes
d. 15 minutes
e. None of the above
30 minutes
A medical technologist performed forward typing, upon viewing the result microscopically there is presence of rouleaux formation, it can be resolve by:
a. DTT
b. 2-mercaptoethanol
c. Washing of RBCs
d. All of the above
Washing of RBCs
Which of the following condition would demonstrate rouleaux formation upon testing:
a. Cord blood sample
b. Patients taking dextran
c. Patients with multiple myeloma
d. All f the above
All f the above
Incomplete washing of RBCs during AHG testing would result into:
a. False negative result
b. Neutralization of AHG reagent
c. False positive result
d. Precipitation of sample
e. A and B
f. B and C
A and B
What is the purpose of adding check cells for negative results in AHG testing:
a. Validate the test
b. Know if AHG reagent is added
c. A and B
d. None of the above
Know if AHG reagent is added
AHG testing incubation can be shortened by adding which of the following potentiator.
a. DTT
b. 2-mercaptoethanol
c. Ficin
d. LISS
e. A and B
LISS
Which of the following reagent can make cross-links between IgG coated RBCs:
a. LISS
b. PEG
c. AHG
d. 22% bovine albumin
AHG
Failure to wash the red cells prior to performing a DAT will lead to:
a. False positives due to complement binding
b. False positives due to nonspecific globulin binding
c. False negatives due to unbound globulin binding
d. False negatives due to failure to remove unbound RBC antigens
e. None of the above
False negatives due to unbound globulin binding
Using the low ionic strength solution medium (LISS) the equilibrium for most antigen antibody reaction is react within ________ minutes.
a. 30
b. 2 – 5
c. 10 – 15
d. 45
10 – 15
A positive AHG phase result in the antibody identification procedure would mean that the antibody/antibodies is/are:
- Warm
- Cold
- Clinically significant
- Not clinically significant
- IgG
- IgM
a. 1, 3,6
b. 2,4,6
c. 1,3,5
d. 1,3 (antibody type is indeterminate)
1,3,5
Reaction medium used in Antihuman globulin test which removes water in order to concentrate the antibodies
present.
a. Albumin
b. DTT
c. Polyethylene glycol
d. LISS
Polyethylene glycol
Which among the following is true regarding the ABO blood group system:
a. ABO antibodies are mostly IgG in nature
b. ABO blood group is the most immunogenic
c. ABO antigens are haptens in nature
d. ABO is ISBT 004
ABO blood group is the most immunogenic
This gene is said to be absent in a Bombay phenotype
a. H gene
b. Le gene
c. ABO gene
d. Z gene
H gene
This antibody is present in Bombay phenotype that is not seen in a type O individual.
a. Anti AB
b. Anti A1
c. Anti H
d. Anti A1B
Anti H
Formation of the ABO antigen on the red cell is controlled by what gene?
a. Se gene
b. H gene
c. Both
d. Neither
H gene
Formation of the ABO antigens on secretions is indirectly controlled by what gene?
a. Se gene
b. H gene
c. Both
d. Neither
Both
The type of antibody that is against an antigen found on the own patients red cells
a. Autoantibody
b. Alloantibody
c. Both
d. Neither
Autoantibody
What blood group would resemble bombay phenotype?
a. O
b. AB
c. A
d. NOTA
O
Which of the following lectins differentiates A1 from A2 cells?
a. Dolichos biflorus
b. Salvia sclarea
c. Glycine soya
d. Ulex europaeus
Dolichos biflorus
Anti H lectin would agglutinate which type of red blood cell:
a. A cell
b. O cell
c. B cell
d. AOTA
AOTA
