M6S3: Perpetuation of Life Genetic Engineering Flashcards
A process by which scientists alter the genetic material of an organism by adding, deleting, or modifying specific genes to produce desired traits
Genetic Engineering
This technology allows for precise manipulation of DNA, enabling the creation of organisms with new or enhanced characteristics
Genetic Engineering
Involves using enzymes and various laboratory techniques to manipulate and isolate DNA segments of interest
Recombinant DNA Technology
James Watson and Francis Crick, with contributions from Rosalind Franklin, discover the double helix structure of DNA
Discovery of DNA Structure (1953)
- Paul Berg creates the first recombinant DNA molecule
- Stanley Cohen and Herbert Boyer insert recombinant plasmid into bacteria, proving foreign DNA can be expressed in living organisms
Birth of Molecular Cloning (1972 - 1973)
Scientists, ethicists, and policymakers discuss the risks and ethical implications of genetic engineering
Asilomar Conference on Recombinant DNA (1975)
Commercialization and Impact (1980s-Present)
- Approval of human insulin produced by genetically modified bacteria
First Application (1982)
Commercialization and Impact (1980s-Present)
- Development of GM crops (e.g., herbicide-resistant soybeans, pest-resistant corn) increases food security
Agriculture
Commercialization and Impact (1980s-Present)
- Production of biopharmaceuticals (e.g., insulin, growth hormones) and advancements in gene therapy
Medicine
What is an example of a biopharmaceutical produced using genetic engineering?
Insulin, growth hormones
Agriculture: Enhanced crop yields, reduced chemical use, improved food security
Medicine: Development of treatments, biopharmaceuticals, gene therapy
Industrial Biotechnology: Production of enzymes, biofuels, and chemicals using GMOs.
Impact: Contributions to sustainability and health
Impact on Biotechnology
Concerns: Potential risks to health and environment, ethical implications
Regulation: Establishment of frameworks to ensure safe use of genetic engineering
Impact: Ongoing discussions about the responsible use of biotechnology
Ethical and Regulatory Considerations
The Process of Genetic Engineering
- Scientists first identify the gene of interest, which could be responsible for a specific trait or function
Gene Identification and Isolation
The Process of Genetic Engineering
- the isolated gene is inserted into a vector, such as a plasmid, which is a small, circular piece of DNA that can replicate independently within a host cell
Gene Cloning
The Process of Genetic Engineering
- the cloned gene is then inserted into the genome of the target organism using techniques such as microinjection, gene gun, or CRISPR-Cas9
Gene Insertion