Lymphocytic Series

Question 1

Lymphoblast

Answer 1

-Cell size: 10-20 um
-N/C ratio 4:1
-Round or oval nucleus
-Central or eccentric nucleus
-Undifferentiated sparse red purple chromatin
-1-2 indistinct nucleoli
-Scanty, often nearly absent,
clear basophilic cytoplasm*
-No granules

Question 2

Identify

Answer 2

Lymphoblast

Question 3

Identify

Answer 3

Lymphoblast

Question 4

Identify

Answer 4

Lymphoblast

Question 5

Prolymphocyte

Answer 5

Cell size: 9 - 18 um
N/C ratio 4:1 (occasionally 3:1)
Round or oval and flat nucleus
Central or eccentric nucleus
Combination of condensed clumped blue-purple chromatin with red-purple parachromatin*
0-1 more distinct nucleoli*
Often scanty, but visable clear basophilic cytoplasm
No granules

Question 6

Identify

Answer 6

Prolymphocyte

Question 7

Identify

Answer 7

Question 8

Lymphocyte

Answer 8

-Cell size: 7-18 um (usually 9-12)
-N/C ratio 4:1 (occasionally 3:1)
-Round or indented nucleus
-Eccentric nucleus often with
scanty cytoplasm
-Homogeneous, coarse blue-purple
nuclear chromatin (smudged)
-Nucleoli usually absent , rarely one seen in mature forms
-Light to dark blue cytoplasm, scanty
to moderate
-Occasionally a few azurophilic granules seen

Question 9

Identify

Answer 9

Arrows pointing at Reactive Lymphs
Above is Monocyte.

Question 10

Can B and T Cells be identified?

Answer 10

NOT until reactive T-cell and Plasma Cell.

Question 11

Plasmablast

Answer 11

-Cell size: 16-18 um
-N/C ratio 4:1
-Round nucleus
-Central or eccentric nucleus
-Pale red- purple, fine stippled chromatin
-Usually 1-3 nucleoli
-Scanty to moderate, paler blue than more mature forms, occasionally perinuclear clear zone
-No granules

Question 12

Identify

Answer 12

Plasmablast

Question 13

Proplasmacyte

Answer 13

Cell size: 15 -25 um
N/C ratio 3:1
Round or oval nucleus
Central or eccentric nucleus
Red- purple, increased granularity of chromatin
Usually 1-3 nucleoli
Moderate, basophilic
cytoplasm, may or may not
have perinuclear clear zone adjacent to nucleus
No granules

Question 14

Identify

Answer 14

Proplasmacyte

Question 15

Plasma Cell

Answer 15

-Cell size: 8 - 20 um
-N/C ratio 2:1 or 1:1
-Round or oval nucleus
-Usually eccentric nucleus
-Blue - purple, dense chromatin w/
large clumps near nuclear margin
-No nucleoli
-Moderate, basophilic
cytoplasm, with perinuclear clear
zone adjacent to nucleus, may contain vacuoles
-No granules (may see Russell Bodies)

Question 16

Russell body

Answer 16

Red or white globular inclusions of Ig in the cytoplasm.

May be so voluminous as to obliterate the other cell structures (mott cell, morula cell or grape cell)

May also be in crystals or rods

Question 17

Identify

Answer 17

Plasma Cell

Question 18

Identify

Answer 18

Plasma Cells

Question 19

Plasma Cells Characteristics

Answer 19

Not normally found in peripheral blood

In bone marrow may comprise 5% of leukocyte population

Plasmacytoid lymphs and plasma cells may occur in blood during long-term infections and chronic allergic syndromes.

An increase in marrow plasma cells or their appearance in the peripheral blood is always cause to consider the possibility of multiple myeloma or plasma cell leukemia

Question 20

Morphological Comparison of
Large Lymphocytes and Monocytes

Answer 20

Question 21

Plasma cell malignancies

Answer 21

-immature plasma cells can be seen in the marrow and/or blood.
-In this pathological plasma cell series, immature B-lymphocyte precursors begin antibody production.

Question 22

Identify

Answer 22

Russell Bodies

Question 23

Identify

Answer 23

Russell Bodies

Question 24

Identify

Answer 24

Russell Bodies

Question 25

Identify

Answer 25

Mott Cell

Question 26

Identify

Answer 26

Mott Cell

Question 27

Flame Cell

Answer 27

Brilliant red staining cytoplasm
(Flame Cell, produces IgA)

Question 28

Identify

Answer 28

Flame Cell

Question 29

Identify

Answer 29

Flame Cell

Question 30

Identify

Answer 30

Flame Cell