Lymphocytic Series Flashcards
Lymphoblast
-Cell size: 10-20 um
-N/C ratio 4:1
-Round or oval nucleus
-Central or eccentric nucleus
-Undifferentiated sparse red purple chromatin
-1-2 indistinct nucleoli
-Scanty, often nearly absent,
clear basophilic cytoplasm*
-No granules
Identify
Lymphoblast
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Lymphoblast
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Lymphoblast
Prolymphocyte
Cell size: 9 - 18 um
N/C ratio 4:1 (occasionally 3:1)
Round or oval and flat nucleus
Central or eccentric nucleus
Combination of condensed clumped blue-purple chromatin with red-purple parachromatin*
0-1 more distinct nucleoli*
Often scanty, but visable clear basophilic cytoplasm
No granules
Identify
Prolymphocyte
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Lymphocyte
-Cell size: 7-18 um (usually 9-12)
-N/C ratio 4:1 (occasionally 3:1)
-Round or indented nucleus
-Eccentric nucleus often with
scanty cytoplasm
-Homogeneous, coarse blue-purple
nuclear chromatin (smudged)
-Nucleoli usually absent , rarely one seen in mature forms
-Light to dark blue cytoplasm, scanty
to moderate
-Occasionally a few azurophilic granules seen
Identify
Arrows pointing at Reactive Lymphs
Above is Monocyte.
Can B and T Cells be identified?
NOT until reactive T-cell and Plasma Cell.
Plasmablast
-Cell size: 16-18 um
-N/C ratio 4:1
-Round nucleus
-Central or eccentric nucleus
-Pale red- purple, fine stippled chromatin
-Usually 1-3 nucleoli
-Scanty to moderate, paler blue than more mature forms, occasionally perinuclear clear zone
-No granules
Identify
Plasmablast
Proplasmacyte
Cell size: 15 -25 um
N/C ratio 3:1
Round or oval nucleus
Central or eccentric nucleus
Red- purple, increased granularity of chromatin
Usually 1-3 nucleoli
Moderate, basophilic
cytoplasm, may or may not
have perinuclear clear zone adjacent to nucleus
No granules
Identify
Proplasmacyte
Plasma Cell
-Cell size: 8 - 20 um
-N/C ratio 2:1 or 1:1
-Round or oval nucleus
-Usually eccentric nucleus
-Blue - purple, dense chromatin w/
large clumps near nuclear margin
-No nucleoli
-Moderate, basophilic
cytoplasm, with perinuclear clear
zone adjacent to nucleus, may contain vacuoles
-No granules (may see Russell Bodies)
Russell body
Red or white globular inclusions of Ig in the cytoplasm.
May be so voluminous as to obliterate the other cell structures (mott cell, morula cell or grape cell)
May also be in crystals or rods
Identify
Plasma Cell
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Plasma Cells
Plasma Cells Characteristics
Not normally found in peripheral blood
In bone marrow may comprise 5% of leukocyte population
Plasmacytoid lymphs and plasma cells may occur in blood during long-term infections and chronic allergic syndromes.
An increase in marrow plasma cells or their appearance in the peripheral blood is always cause to consider the possibility of multiple myeloma or plasma cell leukemia
Morphological Comparison of
Large Lymphocytes and Monocytes
Plasma cell malignancies
-immature plasma cells can be seen in the marrow and/or blood.
-In this pathological plasma cell series, immature B-lymphocyte precursors begin antibody production.
Identify
Russell Bodies
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Russell Bodies
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Russell Bodies
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Mott Cell
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Mott Cell
Flame Cell
Brilliant red staining cytoplasm
(Flame Cell, produces IgA)
Identify
Flame Cell
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Flame Cell
Identify
Flame Cell
