Lymph 1 Flashcards
Give an example of a chronic stimulation of the immune system
H pylori
Sjordren’s syndrome - parotid gland
T cell lymphomas can be driven by?
Coeliac disease
EBV can cause
B cell lymphoma
if you lose T cell function e.g. of
Name three divisions of the lymphoreticular system
Generative tissue (Bone marrow and thymus)
Reactive tissue (devleopment of immune reaction)
Acquired LR tissue (development of local immune reaction)
How can we divide up the cells of the lymphoreticular system?
Lymphocytes and Accessory cells
Give examples of accessory cells
APCs
Macrophages
Connective tissue cells
Name parts of the lymph node where B cells go for maturation
Mantle zone
Germinal zone
Describe where the B cells exist in lymph nodes
Naive unstimulated B cells exist in the dark mantle zone
The centre is the germinal centre and the B cells are interacting with APCs, for activation and proliferation
Interfollicular areas contain T cells and APCs as well as high endothelial vessels
T cells also under APC stimulated activation, selection and proliferation
What do we use to differentiate between
CD 20 - B cells stain positively to CD 20 whereas T cells don’t
What staining is used for T cell lyphomas?
Cell type - CD3, CD5 staining
What staining is used for B cell lyphomas?
CD20
What do you look for in histology?
Sheets or in intrafollicular areas?
Cell distribution
Loss of normal surface proteins
Abnormal expression of proteins
Clonality of B cells - both kappa and lamda like chains
What molecular techniques are used and why?
FISH - to demonstrate chromosomal translocations
PCR - used to confirm the presence of clonal B or T cell receptor or immunoglobulin gene of B cells
FOllicular lymphoma translocation and protein
t14;18 translocation
leads to overexpression of bcl2 protein
Marginal zone lymphoma arise from?
Arise mainly in extranodal sites e.g. gut, lung, spleen
thought to arise
