LT Microscope

Question 1

What are the functions of light microscope components?

Answer 1

• Magnification and resolution
• Focus adjustment (coarse and fine knobs)
• Specimen movement (mechanical stage)

Question 2

Differences between compound and stereoscopic microscopes?

Answer 2

Compound:
- Higher magnification (up to 400X)
- Thin specimen viewing
Stereoscopic:
- 3D images
- Thicker specimen viewing
- Lower magnification (50X)

Question 3

Total magnification calculation?

Answer 3

Ocular magnification × Objective magnification (e.g., 10X × 40X = 400X)

Question 4

Relationship between magnification and working distance?

Answer 4

As magnification ↑, working distance ↓

Question 5

How to calculate field diameter?

Answer 5

Use the equation:
Field Diameter1 × Magnification1 = Field Diameter2 × Magnification2

Question 6

Estimating object size under a microscope?

Answer 6

Measure fraction of field diameter occupied
Multiply field diameter by this fraction

Question 7

Plant vs. animal cells under a microscope?

Answer 7

Plant cells:
- Cell wall
- plasma membrane
- nucleus
- cytoplasm
- Stained with iodine or methylene blue
Animal cells:
- Plasma membrane
- nucleus
- cytoplasm
- Stained with methylene blue

Question 8

Why water for plant cells and saline for animal cells?

Answer 8

Plant cells need water for turgidity
Animal cells need isotonic saline to avoid damage

Question 9

Coarse vs. fine focus knobs?

Answer 9

Coarse Focus: For 4X objective only
Fine Focus: For 10X, 40X, and higher objectives

Question 10

Proper microscope storage?

Answer 10

Lower stage
Set to 4X objective
Turn off light, wrap cords
Store in assigned cabinet slot