Liquids and Solutions 1.1 Flashcards

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1
Q

what do linear dilutions consist of?

A

a range of dilutions that differ by an equal interval

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2
Q

what do log dilutions consist of?

A

a range of dilutions that differ by a constant proportion

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3
Q

what are standard curves used for?

A

to determine an unknown value

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4
Q

what is a buffer?

A

a solution where adding acids or alkalis have little effect on pH

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5
Q

how do buffers work?

A

it allows pH in a reaction mixture to be kept constant

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6
Q

what are colorimeters used for?

A

to quantify concentration and turbidity

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7
Q

what are colorimeters calibrated with and why?

A

they must be calibrated with a blank sample to provide a baseline reading

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8
Q

how does a colorimeters work?

A

light is passed through a sample solution where a detector picks up how much light has been absorbed by the sample or transmitted

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9
Q

what can absorbance determine?

A

concentration of a coloured solution using suitable wavelength filters

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10
Q

what does percentage transmission determine

A

turbidity - cells in suspension

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11
Q

how do linear dilutions work?

A

different volumes of stock solution are added to different solvents

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12
Q

how do log dilutions work?

A

each dilution acts as the stock dilution for subsequent dilution

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