Detecting Proteins using Antibodies 1.1

Question 1

what are immunoassay techniques used for?

Answer 1

to detect and identify specific proteins

Question 2

what do immunoassay techniques use?

Answer 2

stocks of antibodies with the same specificity, known as monoclonal antibodies

Question 3

what is an antibody specific to the protein antigen is linked to?

Answer 3

a ‘chemical label’

Question 4

what is used after SDS-PAGE electrophoresis

Answer 4

western blotting

Question 5

describe western blotting

Answer 5

• separated proteins from the gel are transferred (blotted) onto a solid medium
• proteins can be identified using specific antibodies that have reporter enzymes attached

Question 6

what is ELISA?

Answer 6

an analytical technique which uses antibodies to detect the presence of an antigen in a solution