Linkage, Recombination And Eukaryotic Mapping II Flashcards
What is the function of somatic-cell hybridization?
It is used to determine which chromosome contains a gene of interest.
What can be concluded from a gene product being present in a cell line with an intact chromosome but is missing from a line with chromosome deletion?
The gene for that product must be located in the deleted region.
What can radiation hybrids be useful for?
For determining linkage.
What can deletion mapping be used for?
To determine the chromosmal location of a gene.
When crossing individuals in which one has a deleted chromosal region, a gene of interest may be in a deletion region. If this happens, how much of the progeny will display the mutant phenotype?
1/2
A crossover with a two individuals in which a chromosome has a deleted region, what occurs when a gene of interest is not on the deleted region?
All of the progeny will be wild type.
What is fluoresence in-situ hybridization used for?
It is used to detect small regions of deletions or dupications.
It is used to examine specific regions of the genome in non-dividing tissues.
It is also used to map genes.
What is fluorescence-in-situ-hybridization?
A technique in which a DNA probe containing one or more genes is labeled with a fluorescent dye, and then hybridized to interphase or metaphase cells.
What are PRader-willi syndrome and angelman syndrome caused by?
Deletion of part of a chromosomem.
True or false: levels of recombination vary widely.
True. They vary widely:
Among species
Among chromosomes of a single species
Among males and females.
True or false: recombination rates vary across a chromosome.
True
What should a model genetic organism have?
Easily defined charactersitics.
What are used to determine the physical positions of genes on particular chromosomes?
Physical mapping methods. This includes:
Somatic-cell hybridization
Deletion mapping
Physical mapping through molecular analysis.
