Lineage Specific Hematopoesis Flashcards
During the maturation of a normal red blood cell, there will be normal changes that will happen or occur in terms of the:
Size
Nucleus
Cytoplasm
Chromatin
Granules
Fill in the blanks:
The cytoplasm of an immature cell is usually _____ or _____ due to RNA content.
Blue
Basophilic
True or False
The more mature the cell, the more basophilic because of the less RNA.
False
The more mature the cell, the less basophilic because of the less RNA.
Fill in the blank:
In _____, the cytoplasm contains granules.
Myeloid
The _____ is also known as acidophilic or eosinophilic granules.
Red (Acidic) Dye
The _____ is also known as the basophilic granules.
Blue (Basic) Dye
Combined basic and acidic dye.
Neutrophilic Granules
Special feature of the maturation of erythrocytes cells.
Elaboration of Hemoglobin
There are how many Series of Maturation?
6 Series of Maturation
Fill in the blanks:
At the mature stage of the hemoglobin, the nucleus is apparently no longer necessary and eliminated by _____ or _____.
Nucleolysis
Extursion
Fill in the blank:
A good example of nucleolysis is _____.
Erythrocytes
Fill in the blank:
In the nuclear changes, the immature nucleus is round or oval and the nuclear chromatin is very delicate, fine, and linear and is called _____.
Euchromatin
Give the characteristics of Euchromatin:
Very delicate
Fine
Linear
Pale Blue
Uncondensed
Fill in the blank:
As the cells matures, chromatin stands increasingly coarse and clumped _____.
Heterochromatin
RBC Inclusion Bodies:
Pappenheimer
Howell Jolly Bodies
Heinz Bodies
WBC Inclusion Bodies:
Alder-Reilly
If the nucleus is hyposegmented, what condition is associated?
Pelger Huet Anomaly (Lack of Lamin B)
In the erythrocyte series, what is larger in terms of size?
Megaloblasts are larger than normal mature erythrocytes
3 Stem Cells:
Totipotent Stem Cells
Pluripotent Stem Cells
Multipotent Stem Cells
First recognizable cell in the series of granular poesis is the _____.
Myeloblasts
2 progenitors in the Human Hematopoesis:
Common Myeloid Progenitors
Common Lymphoid Progenitors
First recognizable erythroid precursor.
Pronormoblast (Rubriblast)
Basophilic Normoblast (Prorubricyte) is mistaken as Pronormoblast (Rubriblast). To differentiate, what are the criteria in distinguishing which of which?
- Prorubricyte has coarse chromatins
- Absence of nucleoli
First stage of Hemoglobin synthesis in Erythropoiesis.
Basophilic Normoblast (Prorubricyte)
Polychromatic (Polychromatophilic) Normoblast
(Rubricyte) can be mistaken as Lymphocytes. To differentiate, what are the criteria?
- Lymphocytes have crushed velvet nucleus and cytoplasm is sky blue
- In Rubricyte, nucleus is checker board and cytoplasm is muddy or gray
It is the last stage in the Erythropoiesis where the mitosis stops.
Polychromatic (Polychromatophilic) Normoblast
(Rubricyte)
Orthochromic Normoblast (Metarubricyte) is also known as _____
Late Normoblast
Last stage to synthesize Hemoglobin in Erythropoiesis.
Reticulocyte
Last stage in bone marrow before release to the blood in Erythropoiesis.
Reticulocyte
Best indicators of bone marrow functions.
Reticulocyte Count
This stain is used to visualize Reticulocytes.
Supravital Stain
Reference Range of Reticulocyte in Adults.
0.5% to 1.5%
Reference Range of Reticulocyte in Newborn.
2% to 6%
Last stage in the Erythropoiesis.
Erythrocyte
If an Erythrocyte has no central pallor, it indicates what issue?
Spherocytes
Enumerate the Normoblastic Nomenclature for Erythroid Precursors:
Pronormoblast
Basophilic Normoblast
Polychromatic Normoblast
Orthochromatic Normoblast
Reticulocyte
Erythrocyte
Enumerate the Rubriblastic Nomenclature for Erythroid Precursors:
Rubriblast
Prorubricyte
Rubricyte
Metarubricyte
Reticulocyte
Erythrocyte
Enumerate the Erythroblastic Nomenclature for Erythroid Precursors:
Proerythroblast
Basophilic Erythroblast
Polychromic Erythroblast
Orthochromic Erythroblast
Reticulocyte
Erythrocyte
Identify:
Pronormoblast Size
12-20 um
Identify:
Pronormoblast Nucleus to Cytoplasm Ratio
8:1
Identify:
Pronormoblast Nucleoli
1-2
Identify:
Pronormoblast Percentage in Bone Marrow
1%
Identify:
Pronormoblast Transit Time in Bone Marrow
24
Identify:
Pronormoblast Division
Mitosis (2)
Identify:
Basophilic Normoblast Size
10-15um
Identify:
Basophilic Normoblast Nucleus to Cytoplasm Ratio
6:1
Identify:
Basophilic Normoblast Nucleoli
0-1
Identify:
Basophilic Normoblast Percentage in Bone Marrow
1-4%
Identify:
Basophilic Normoblast Transit Time in Bone Marrow
24
Identify:
Basophilic Normoblast Division
Mitosis (4)
Identify:
Polychromic Normoblast Size
10-12 um
Identify:
Polychromic Normoblast Nucleus to Cytoplasm Ratio
4:1
Identify:
Polychromic Normoblast Nucleoli
0
Identify:
Polychromic Normoblast Percentage in Bone Marrow
10-20%
Identify:
Polychromic Normoblast Transit Time in Bone Marrow
24
Identify:
Polychromic Normoblast Division
Mitosis (16)
Identify:
Orthochromic Normoblast Size
8-10 um
Identify:
Orthochromic Normoblast Nucleus to Cytoplasm Ratio
1:2
Identify:
Orthochromic Normoblast Nucleoli
0
Identify:
Orthochromic Normoblast Percentage in Bone Marrow
5-10%
Identify:
Orthochromic Normoblast Transit Time in Bone Marrow
48
Identify:
Orthochromic Normoblast Division
Not applicable
Identify:
Reticulocyte Size
8-10 um
Identify:
Reticulocyte Nucleus to Cytoplasm Ratio
Not applicable
Identify:
Reticulocyte Nucleoli
0
Identify:
Reticulocyte Percentage in Bone Marrow
1%
Identify:
Reticulocyte Transit Time in Bone Marrow and in Peripheral Blood
Bone Marrow = 1-2 days
Peripheral Blood = 1 day
Identify:
Red Blood Cell Size
Diameter = 6-8 um
Thickness = 1.5-2.5 um
Identify:
Red Blood Cell Nucleus to Cytoplasm Ratio
Not applicable
Identify:
Red Blood Cell Nucleoli
0
Identify:
Red Blood Cell Percentage in Bone Marrow
0
Identify:
Red Blood Cell Transit Time in Bone Marrow and in Peripheral Blood
Bone Marrow = 0
Peripheral Blood = 120 days
Identify:
Red Blood Cell Division
Not applicable
Enumerate the Granulopoiesis in chronological order:
Myeloblast
Promyelocyte
Myelocyte
Metamyelocyte
Non-segmented Granulocyte
Segmented Granulocyte
The first or earliest recognizable granulocyte precursor
Myeloblasts
3 Types of Myeloblasts:
Type I - Nucleus is open
Type II - Presence of primary granules
Type III - Darker in color
