Leukopoor Red Blood Cells Flashcards
What is the shelf life of leukopoor red blood cells in a closed system?
> The shelf life of leukopoor red blood cells in a closed system is the same as packed red blood cells.
What is the shelf life of leukopoor red blood cells in an open system?
> Leukopoor red blood cells have a shelf life of 24 hours.
How many Celcius in the storage temp for Leukopoor Red Blood Cells?
> 1-6ºC
Give the contents about Leukopoor Red Blood Cells
> 5 x 106 residual White Blood Cell (WBC)
Give the indications about Leukopoor Red Blood Cells
> Anemia with history of febrile reactions; to decrease alloimmunization to
WBC or HLA antigens or CMV transmission
Enumerate the 3 means of Leukocyte Removal
- Centrifugation
- Washing procedures using saline or glycerol
- Mechanical separation using leukoreduction filters
Enumerate the 3 leukoreduction filters
- First generation filters-170 um
- Second generation filters-20-40 um
- Third generation filters (3-log filter)
Where does CMV reside in the body, and how does it typically manifest in healthy donors?
> CMV resides in WBC monocytes and macrophages and often manifests as a self-limiting virus, remaining asymptomatic among carriers who appear healthy.
What is the method used for leukocyte reduction during centrifugation in an open system?
> Leukocyte reduction during centrifugation in an open system is achieved by selectively removing the buffy coat layer, which is rich in white blood cells.
Washing procedures (open system) using __________ excess White Blood Cell (WBC) are removed
> Saline or glycerol
Mechanical separation using __________ the best method in reducing the WBC population
> Leukoreduction filters
Give the 3 leukoreduction filters.
- First generation filters-170 um the one used
- Second generation filters-20-40 um
- Third generation filters (3-log filter)
Enumerate the cells/components in the blood filtered in leukoreduction filters
- Fibrin clot
- Giant platelets
- Microcytes- large red blood cells
- Large white blood cells fragments
- Most of the WBC
Enumerate the cells pass through the filter
- Normal platelets
- Normal RBC
- WBC same size with normal RBC
Give the Centrifugation Procedure
- Invert and Centrifuge
- Remove the Red Cells
Leukocite-Poor Blood Storage 35 days at 4°C