Leukopoor Red Blood Cells Flashcards

1
Q

What is the shelf life of leukopoor red blood cells in a closed system?

A

> The shelf life of leukopoor red blood cells in a closed system is the same as packed red blood cells.

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2
Q

What is the shelf life of leukopoor red blood cells in an open system?

A

> Leukopoor red blood cells have a shelf life of 24 hours.

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3
Q

How many Celcius in the storage temp for Leukopoor Red Blood Cells?

A

> 1-6ºC

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4
Q

Give the contents about Leukopoor Red Blood Cells

A

> 5 x 106 residual White Blood Cell (WBC)

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5
Q

Give the indications about Leukopoor Red Blood Cells

A

> Anemia with history of febrile reactions; to decrease alloimmunization to
WBC or HLA antigens or CMV transmission

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6
Q

Enumerate the 3 means of Leukocyte Removal

A
  1. Centrifugation
  2. Washing procedures using saline or glycerol
  3. Mechanical separation using leukoreduction filters
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7
Q

Enumerate the 3 leukoreduction filters

A
  1. First generation filters-170 um
  2. Second generation filters-20-40 um
  3. Third generation filters (3-log filter)
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8
Q

Where does CMV reside in the body, and how does it typically manifest in healthy donors?

A

> CMV resides in WBC monocytes and macrophages and often manifests as a self-limiting virus, remaining asymptomatic among carriers who appear healthy.

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9
Q

What is the method used for leukocyte reduction during centrifugation in an open system?

A

> Leukocyte reduction during centrifugation in an open system is achieved by selectively removing the buffy coat layer, which is rich in white blood cells.

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10
Q

Washing procedures (open system) using __________ excess White Blood Cell (WBC) are removed

A

> Saline or glycerol

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11
Q

Mechanical separation using __________ the best method in reducing the WBC population

A

> Leukoreduction filters

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12
Q

Give the 3 leukoreduction filters.

A
  1. First generation filters-170 um the one used
  2. Second generation filters-20-40 um
  3. Third generation filters (3-log filter)
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13
Q

Enumerate the cells/components in the blood filtered in leukoreduction filters

A
  1. Fibrin clot
  2. Giant platelets
  3. Microcytes- large red blood cells
  4. Large white blood cells fragments
  5. Most of the WBC
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14
Q

Enumerate the cells pass through the filter

A
  1. Normal platelets
  2. Normal RBC
  3. WBC same size with normal RBC
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15
Q

Give the Centrifugation Procedure

A
  1. Invert and Centrifuge
  2. Remove the Red Cells

Leukocite-Poor Blood Storage 35 days at 4°C

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