Frozen< Thawed< Deglycerolized RBC Flashcards
Shelflife of FROZEN< THAWED< DEGLYCEROLIZED RBC and how many hours does Deglycerolized?
- Frozen – 10 years
- Deglycerolized – 24 hours
How many Celcius in the storage temp for FROZEN< THAWED< DEGLYCEROLIZED RBC in Freezing time?
- 65ºC (High Glycerol-40%)
- 120ºC (Low Glycerol-20%)
- 65ºC (using 79%glycerol with dextrose fructose and EDTA)
How many Celcius in Deglycerolizing Process
> 1-6ºC
Why might FROZEN, THAWED, and DEGLYCEROLIZED RBCs be used, and what are their indications?
- Used for anemia treatment and long-term storage purposes.
- They are indicated for “rare” blood units and autologous units.
Useful for Autologous donation transfusion which has rare phenotype such as Bombay negative
> FROZEN RBC- “GLYCEROLIZED / CRYOPROTECTED RBC”
What is the purpose of freezing RBCs with glycerolization or cryoprotection?
> Freezing RBCs with glycerolization or cryoprotection is done to prolong their storage life.
What are “GLYCEROLIZED / CRYOPROTECTED RBC” units, and how long is their viability and expiration date compared to regular donor blood units?
- “GLYCEROLIZED / CRYOPROTECTED RBC” units, also known as Frozen RBCs, are red blood cells that have been preserved using glycerol and a cryoprotectant
- These units can remain viable for up to 10 decades, giving them a much longer expiration date
What is the process involved in creating “Frozen RBC -Glycerolized / Cryoprotected RBC”?
- By adding the first cryoprotective agent to the donor unit before storing the red blood cells (RBC) at an extremely low temperature.
What is the purpose of using a penetrating cryoprotective agent that utilizes a low molecular weight agent?
- commonly employed in laboratory settings to prepare red blood cells (RBCs) before freezing.
What is the most penetrating agent?
> Glycerol
What are the benefits of using a penetrating agent in extreme cold/low-temperature storage for cells?
The penetrating agent serves several key functions:
1. Protects the cell membrane from extreme
cold/ low temp storage
2. Prevents ICE CYRSTAL FORMATION in the cell
3. Prevents the loss of water dehydration of
cell when in cold temperature
How does the storage of a penetrating cryoprotective agent relate to the concentration of glycerol, and how long is it effective for?
- Depends on the concentration of glycerol. This storage method remains effective for up to one decade.
How many percentage in high concentration glycerol more recommended because there is
no liquid nitrogen and the concentration is
not low?
> 40%
Freezing req for RBC under high concentration glycerol __________ degrees which is mehanical freezer.
> 65 degrees
How many percentage in low concentration glycerol under types of agents of cryoprotective RBC?
> 20% but there is a specific requirement of Liquid nitrogen
Very low concentration will result in cell lysis
How many degrees does req for RBC under Low Concentration GLYCEROL?
> 120 degree
What freezer does in Low concentration glycerol?
> Liquid nitrogen freezer
What freezer does in high concentration glycerol?
> Mechanical Freezer
What will happen if frozen RBC is needed?
> Thawing Process is Performed
What is the process before the unit is transfused under Frozen RBC- “Glycerolized/Cryoprotected RBC”?
> Removed first the glycerol “DEGLYCEROL
PROCESS”= removal of glycerol in the donor unit
Used washed solution which has 3 washed procedure
to removed glycerol from the unit.
> DEGLYCEROLIZING PROCESS
Hypertonic saline solution in __________ until isotonic level is attained.
> Decreasing concentration
How many hours does every after 3 washed placed the unit in ref temperature for
the meantime and transfused?
> Within 24 hrs
Enumerate the 3 washed steps in deglycerolizing process.
- WASHED THE THAWED with 12% saline solution which is hypertonic
solution which removed the glycerol without affecting the viability of the
RBC (It will not shrink it will maintain) - 2nd washed RBC with 1.6% saline solution “hypertonic” = facilitate the
complete removal of glycerol without effecting the viability of RBC. - 3RD washed 0.2% dextrose in normal saline solution= Isotonic solution prior
to transfusion which is add in NSS
What is the process of Hypertonic solution in DEGLYCEROLIZING PROCESS?
> Remove glycerol to cell membrane
What is the use of Physiologic solution Dextrose?
> it is used to provide additional source
of ATP of the cells
What Normal Saline Solution-Isotonic maintaining of?
> Maintain the morphology of the cell prior to blood transfusion
- High molecular weight agent
- “HES” HYDOXYL ETHYL STARCH- commonly used
- CAN’T PASS TRHOUGH THE CELL MEMBRANE
> 2nd- non penetrating cryoprotective agent
In the 2nd non penetrating cryoprotecttive agent Sole Action protects ____________.
> Protects the cell surface from extremely low
temperature
