Leucine - Urea (RVSP)

Question 0

Rgnt for Salkowski’s Test

Answer 0

10% Copper sulfate

Question 1

Tests for Leucine

Answer 1

• Salkowski’s Test
• Thin Layer Chromatography - Paper Chromatography
• Microscopic examination

Question 2

Postive for Salkowski’s test

Answer 2

Blue color

Question 3

Positive for microscopic examination for leucine

Answer 3

Oily looking spheres or crystals

Question 4

Tests for Leucine, Isoleucine, Valine (MSUD)

Answer 4

FeCl3

Nitroso-naphthol test

Question 5

Rgnt for FeCl3 (MSUD)

Answer 5

10% FeCl3

Question 6

Rgnt for Nitroso-naphthol test (for MSUD)

Answer 6

HNO3
Sodium Nitrite
Nitroso-naphthol

Question 7

Positive for FeCl3 (MSUD)

Answer 7

Green-gray

Question 8

Positive for Nitroso-naphthol test (MSUD)

Answer 8

Red

Question 9

Tests for Mucopolysaccharide

Answer 9

• Cetyltrimethylammonium bromide (CTAB)

- MPS paper test

Question 10

Rgnt for Cetyltrimethylammonium bromide (CTAB)

Answer 10

5% CTAB

Question 11

Rgnt for MPS Paper test

Answer 11

HAc
Methanol
MPS Paper

Question 12

Positive for CTAB

Answer 12

White turbidity

Question 13

Positive for MPS Paper test

Answer 13

Blue

Question 14

Tests for Melanin

Answer 14

Bromine water test
FeCl3 test
Thormahlen's test
Blackberg and Wagner's
Ehrlich's reaction

Question 15

Rgnt for Bromine water test

Answer 15

Bromine water

Question 16

Rgnt for FeCl3 test (Melanin)

Answer 16

10% FeCl3

Question 17

Rgnt for Thormahlen’s test

Answer 17

Sodium nitroprusside
NaOH
Glacial HAc

Question 18

Rgnt for Backberg and Wagner’s

Answer 18

Potassium persulfate
Methyl alcohol
Ether

Question 19

Rgnt for Ehrlich’s reaction

Answer 19

Ehrlich’s reagent (PDAB)

Question 20

Positive for bromine water test

Answer 20

Yellow to black ppt

Question 21

Positive for FeCl3 (melanin)

Answer 21

Gray to black ppt

Question 22

Positive for Thormahlen’s test

Answer 22

Green to black ppt

Question 23

Positive for Blackberg and Wagner’s

Answer 23

Brown to black ppt

Question 24

Positive for Ehrlich's reaction (melanin)

Answer 24

Red

Question 25

Tests for Indican

Answer 25

Obermayer's test Jaffe's test Jolle's test Sharlit test

Question 26

Rgnt for Obermayer's test

Answer 26

Obermayer's reagent HCl FeCl3

Question 27

Rgnt for Jaffe's test

Answer 27

HCl Cloroform Calcium hypochlorite

Question 28

Rgnt for Jolle's test

Answer 28

Lead acetate 10% alcohol thymol Obermayer's reagent Chloroform

Question 29

Rgnt for Sharlit test

Answer 29

Potassium persulfate Thymol Alcohol TCA

Question 30

Positive for Obermayer's test

Answer 30

Blue chloroform layer

Question 31

Positive for Jolle's Test

Answer 31

Violet chloroform layer

Question 32

Positive for Jaffe's Test

Answer 32

Blue chloroform layer

Question 33

Positive for Sharlit test

Answer 33

Blue color

Question 34

Tests for 5-HIAA

Answer 34

Ehrlich's test Sjordsma Reaction FeCl3 test Nitroso-naphthol test

Question 35

Reagent for Ehrlich's test (5-HIAA)

Answer 35

Ehrlich's reagent

Question 36

Rgnt for Sjordsma reaction

Answer 36

Nitrous acid

Question 37

Rgnt for FeCl3 test (5-HIAA)

Answer 37

10% FeCl3

Question 38

Reagent for Nitroso-naphthol test (5-HIAA)

Answer 38

HNO3 Sodium nitrite Nitroso-naphthol

Question 39

Positive for Ehrlich's test (5-HIAA)

Answer 39

Blue color

Question 40

Positive for Sjordsma reaction

Answer 40

Purple

Question 41

Positive for FeCl3 Test (5-HIAA)

Answer 41

Blue-green

Question 42

Positive for Nitroso-naphthol test (5-HIAA)

Answer 42

Violet

Question 43

Test for Urea

Answer 43

Hypobromite test

Question 44

Rgnt for Hypobromite test

Answer 44

NaOH Pure bromine Potassium bromide

Question 45

Positive for Hypobromite test

Answer 45

Evolution of nitrogen gas