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Clinical Microscopy > Leucine - Urea (RVSP) > Flashcards

Leucine - Urea (RVSP) Flashcards

0
Q

Rgnt for Salkowski’s Test

A

10% Copper sulfate

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1
Q

Tests for Leucine

A
  • Salkowski’s Test
  • Thin Layer Chromatography - Paper Chromatography
  • Microscopic examination
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2
Q

Postive for Salkowski’s test

A

Blue color

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3
Q

Positive for microscopic examination for leucine

A

Oily looking spheres or crystals

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4
Q

Tests for Leucine, Isoleucine, Valine (MSUD)

A

FeCl3

Nitroso-naphthol test

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5
Q

Rgnt for FeCl3 (MSUD)

A

10% FeCl3

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6
Q

Rgnt for Nitroso-naphthol test (for MSUD)

A

HNO3
Sodium Nitrite
Nitroso-naphthol

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7
Q

Positive for FeCl3 (MSUD)

A

Green-gray

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8
Q

Positive for Nitroso-naphthol test (MSUD)

A

Red

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9
Q

Tests for Mucopolysaccharide

A
  • Cetyltrimethylammonium bromide (CTAB)

- MPS paper test

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10
Q

Rgnt for Cetyltrimethylammonium bromide (CTAB)

A

5% CTAB

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11
Q

Rgnt for MPS Paper test

A

HAc
Methanol
MPS Paper

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12
Q

Positive for CTAB

A

White turbidity

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13
Q

Positive for MPS Paper test

A

Blue

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14
Q

Tests for Melanin

A 
Bromine water test
FeCl3 test
Thormahlen's test
Blackberg and Wagner's
Ehrlich's reaction
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15
Q

Rgnt for Bromine water test

A

Bromine water

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16
Q

Rgnt for FeCl3 test (Melanin)

A

10% FeCl3

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17
Q

Rgnt for Thormahlen’s test

A

Sodium nitroprusside
NaOH
Glacial HAc

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18
Q

Rgnt for Backberg and Wagner’s

A

Potassium persulfate
Methyl alcohol
Ether

19
Q

Rgnt for Ehrlich’s reaction

A

Ehrlich’s reagent (PDAB)

20
Q

Positive for bromine water test

A

Yellow to black ppt

21
Q

Positive for FeCl3 (melanin)

A

Gray to black ppt

22
Q

Positive for Thormahlen’s test

A

Green to black ppt

23
Q

Positive for Blackberg and Wagner’s

A

Brown to black ppt

24
Q

Positive for Ehrlich’s reaction (melanin)

A

Red

25
Q

Tests for Indican

A

Obermayer’s test
Jaffe’s test
Jolle’s test
Sharlit test

26
Q

Rgnt for Obermayer’s test

A

Obermayer’s reagent
HCl
FeCl3

27
Q

Rgnt for Jaffe’s test

A

HCl
Cloroform
Calcium hypochlorite

28
Q

Rgnt for Jolle’s test

A

Lead acetate
10% alcohol thymol
Obermayer’s reagent
Chloroform

29
Q

Rgnt for Sharlit test

A

Potassium persulfate
Thymol
Alcohol TCA

30
Q

Positive for Obermayer’s test

A

Blue chloroform layer

31
Q

Positive for Jolle’s Test

A

Violet chloroform layer

32
Q

Positive for Jaffe’s Test

A

Blue chloroform layer

33
Q

Positive for Sharlit test

A

Blue color

34
Q

Tests for 5-HIAA

A

Ehrlich’s test
Sjordsma Reaction
FeCl3 test
Nitroso-naphthol test

35
Q

Reagent for Ehrlich’s test (5-HIAA)

A

Ehrlich’s reagent

36
Q

Rgnt for Sjordsma reaction

A

Nitrous acid

37
Q

Rgnt for FeCl3 test (5-HIAA)

A

10% FeCl3

38
Q

Reagent for Nitroso-naphthol test (5-HIAA)

A

HNO3
Sodium nitrite
Nitroso-naphthol

39
Q

Positive for Ehrlich’s test (5-HIAA)

A

Blue color

40
Q

Positive for Sjordsma reaction

A

Purple

41
Q

Positive for FeCl3 Test (5-HIAA)

A

Blue-green

42
Q

Positive for Nitroso-naphthol test (5-HIAA)

A

Violet

43
Q

Test for Urea

A

Hypobromite test

44
Q

Rgnt for Hypobromite test

A

NaOH
Pure bromine
Potassium bromide

45
Q

Positive for Hypobromite test

A

Evolution of nitrogen gas

Clinical Microscopy (11 decks)

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