Bile Acids - 17-Hydrocorticoids (FSKP) Flashcards

0
Q

Rgnt for oliver test

A

Peptone
Sal. Acid
HAc

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1
Q

Tests for Bile acids

A

Oliver test
Hay test
Pettenkofer test

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2
Q

Rgnt for Hay test

A

Sulfur powder

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3
Q

Rgnt for Pettenkofer test

A

Sucrose

H2SO4

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4
Q

Result for oliver test

A

Milky turbidity

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5
Q

Result for Hay test

A

Sulfur granule sink

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6
Q

Result for Pettenkofer test

A

Red ring

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7
Q

Test for Hemosiderin

A

Perl’s reaction

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8
Q

Rgnt for Perl’s reaction

A

Potassium ferrocyanide

HCl

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9
Q

Result for Perl’s reaction

A

Prussian blue reaction

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10
Q

Tests for Sulfonamide

A

Lignin or Wood Fiber test

Ehrlich Test

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11
Q

Rgnt for Lignin or wood fiber test

A

10% HCl

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12
Q

Rgnt for Ehrlich’s test

A

Ehrlich’s reagent

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13
Q

Result for Lignin or wood fiber test

A

Yellow to orange

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14
Q

Result for Ehrlich’s test (sulfonamides)

A

Canary yellow to greenish yellow

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15
Q

Tests for Calcium

A

Sulkowitch Test

Barney’s test

16
Q

Rgnt for Sulkowitch test

A

Sulkowitch reagent

  • oxalic acid
  • ammonium oxalate
  • glacial HAc
17
Q

Rgnt for Barney’s test

A

Sulkowitch reagent

18
Q

Result for Sulkowitch test

A

Turbidity or formation of precipitate

19
Q

Result for Barney’s test

A

Turbidity or formation of precipitate

20
Q

Tests for chloride

A

Fantus test

Schales and Schales test

21
Q

Rgnt for Fantus test

A

Silver nitrate

Potassium chromate

22
Q

Rgnt for Schales and Schales test

A

Mercuric nitrate

Diphenylcarbazone indicator

23
Q

Result for Fantus test

A

Red end point

24
Q

Result for Schales and Schales test

A

Pink to violet endpoint

25
Q

Test for 17-ketosteroids

A

Zimmerman reaction

26
Q

Rgnt for zimmerman reaction

A

Ethylene dichloride meta-dinitrobenzene

27
Q

Result for zimmerman reaction

A

Red color

28
Q

Test for 17-Hydrocorticoids

A

Porter Silber Reaction

29
Q

Rgnt for Porter Silber reaction

A

Methylene chloride
Phenylhydrazine
HCl
H2SO4

30
Q

Result for Porter Silber reaction

A

Yellow color