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Clinical Bacteriology > Lesson 6 > Flashcards

Lesson 6 Flashcards

1
Q

Are growth of microorganism on a culture medium

A

Cultures

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2
Q

Is a liquid, semi-solid or solid medium utilized to observe growth patterns of microorganism as well as for transport and storage.

A

Culture Medium

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3
Q

Culture composed of only one specie

A

Pure Culture

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4
Q

Culture composed of more than one specie

A

Mixed Culture

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5
Q

– Is composed of several species
contained in a separate culture
medium (one species/culture
medium).
– It is used for academic and
industrial purposes.

A

Stock Culture

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6
Q

Colonies may be described as being
large, medium, small, or pinpoint.

A

Size

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7
Q

Refers to the edge of colonies

A

Form or Margin

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8
Q

■ Determined by tilting culture plate and looking at the
side of the colony
■ Raised, convex, flat, umbilicate, or umbonate

A

Elevation

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9
Q

May be described as transparent, translucent, opaque.

A

Density

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10
Q

Used to describe a particular colony genus in genera

A

Color

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11
Q

Determined by touching a colony with a sterile loop

A

Consistency

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12
Q

Inherent characteristic of specific organisms

A

Pigment

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13
Q

What is the distinctive odor of staphylococcus aureus?

A

Old Sock

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14
Q

Vinelike growth in media

A

Streamers

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15
Q

Overall cloudiness of liquid

A

Turbidity

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16
Q

It contains 0% agar

A

Liquid Medium

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17
Q

It contains 0.5 to 1% agar

A

Semi-Solid Medium

18
Q

It contains 2-3% agar

A

Solid Medium

19
Q

Agar will melt at what temperature?

A

80 to 90°C

20
Q

Agar will solidify at what temperature?

A

40 to 50°C

21
Q

Cooling temp for distribution of agar into plates

A

55-60°C

22
Q

• It is a medium where all the
components are known to the
user.
• It is use for research purposes.

A

Synthetic or Defined Medium

23
Q

• It is composed of some unknown
substances (peptones, meat and
yeast extracts).
• It is very useful for isolation of
bacteria.
• examples: nutrient broth, TSB
and MacConkey agar

A

Non-Synthetic or Complex Medium

24
Q

• It used for obligate intracellular
bacteria (Rickettsia, Chlamydia).
• Examples: W138, HELA 229 cells
and Mc Coy cells

A

Tissue Culture Medium

25
Q

Are routinely used in the laboratory and without added supplement

A
  1. Simple Media/General Purpose Media/Supportive Media
26
Q

■ It is used to propagate the growth of certain group of organisms.
■ They contain specific nutrients; liquid-type media.
■ It can also be used as supplement to agar plates.
■ Examples: Alkaline Peptone Water, Selenite F, Thioglycolate, tetrathionate, Gram- negative (GN)
broth, Lim broth (group B streptococci)

A

Enrichment Broth

27
Q

■ These are media with added supplements such as blood, vitamins and yeast extract, necessary
for the growth of fastidious organisms.
■ These are solid-type media

A

Enriched Media/Nonselective Media

28
Q

Standard nonselective culture medium

A

BAP

29
Q

Has horse blood is used for the isolation of Hemophilus

A

CAP

30
Q

These are media that allow visualization of
metabolic differences between groups of
bacteria.
■ Examples: MacConkey, BAP, EMB, and HEA

A

Differential Media

31
Q

“Greenish” zone of hemolysis

A

ALPHA

32
Q

“Clear” zone of hemolysis

A

Beta

33
Q

Means No Hemolysis

A

Gamma

34
Q

These are media incorporated with antibiotics, dyes or chemicals to
inhibit the growth of other organisms while promoting the growth of
the desired organism.

A

Selective Media

35
Q

contains bile salt and dyes (acid fuchsin)
for non-enteric pathogens; it inhibits the normal
flora of the lower GIT.

A

HE Agar

36
Q

It contains 0.25% Sodium Deoxycholate salt and sodium thiosulfate to differentiate Salmonella (red with black centers) from Shigella (red or clear colonies).

A

XLD Agar

37
Q

Inhibitory to Gram-positive Bacteria

A
  1. Crystal/Gentian Violet
  2. Basic Fuchsin
  3. Bile Salt
38
Q

Inhibitory to Gram-negative Bacteria

A
  1. Potassium Tellurite
  2. Sodium Azide
39
Q

Inhibitory to Swarming Bacteria

A
  1. Alcohol
  2. Chloral Hydrate
40
Q

Is used to isolate bacteria with specific growth requirements.

A

Special Media

Clinical Bacteriology (9 decks)

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