LESSON 2: APHASMID NEMATODA (ADENOPHOREA) Flashcards
What is the common name of Trichinella spiralis?
Trichina worm
Trichinella spiralis:
a. What is the Infective stage
b. Main Habitat
c. Final Host
d. (T/F) Intermediate hosts of T. spiralis same animal as the final host
a. Encysted larva
b. small intestine, skeletal muscles (larva)
c. hogs, rats, man
d. TRUE
Trichinella spiralis:
First found in the muscle of patient that was autopsied by __________ in _______ in 1828
peacock in London in 1828
Trichinella Spiralis:
In 1835 ______ was the first investigator to describe and name the insisted larvae
Richard Owen
3 sub spp. that can infect a man in Trichinella Spiralis:
a. ______________ – temperate regions
b. ______________ – Artic region (artic bears)
c. ______________ – found in Africa (African
predators and scavengers)
a. Trichinella spiralis spiralis
b. Trichinella spiralis nativa
c. Trichinella spiralis nelson
What are the diseases when a person is infected with T. spiralis?
Trichinosis
Trichiniasis
Trichinellosis
Trichinella spiralis: LARVA
a. at birth: _________ & highly coiled
b. it can reach _________ when it enters a _________
c. has ________ -like burrowing tip at its tapering ________
d. grows rapidly about ____ mm
e. the digestive tract of a mature larvae insisted in a muscle fiber resembles that of an ________
f. the reproductive organ of the T. spiralis is __________ developed, but even then it is already possible to identify the sex of the parasite whether it is a male or a female
a. at birth: 80-120 µm
b. it can reach 900-1000 µm when it enters a muscle fiber
c. has spear-like burrowing tip at its tapering anterior end
d. grows rapidly about 1 mm
e. the digestive tract of a mature larvae insisted in a muscle fiber resembles that of an adult worm
f. the reproductive organ of the T. spiralis is not yet fully developed, but even then it is already possible to identify the sex of the parasite whether it is a male or a female
Trichinella spiralis: ADULTS
Rarely seen in _______ or _____________
stool or any material
T. spiralis: FEMALE
a. Measures __ mm x __ mm
b. Posterior end: _______
anterior fifth: with _______
c. Has a ______ in the ______ part of the body
d. It has an ______ , a ______ , a uterus, a ______ and a ______
e. (T/F): Smaller than males
f. LARVIPAROUS – lay ________
g. Can live for _____ which capable of producing ________ or more in her life time
a. Measures 3.5 mm x 0.06 mm
b. Posterior end: bluntly rounded; anterior fifth: with single vulva
c. Has a single ovary in the posterior part of the body
d. It has an oviduct, a seminal receptacle, a uterus, a vagina and a vulva
e. FALSE - Larger than males
f. LARVIPAROUS – lay larvae
g. Can live for 30 days which capable of producing 1500 larvae or more in her life time
T. spiralis: MALES
a. Measures __ mm x __ mm
b. posterior end: ______________ with two lobular ______________
c. it has a ______________ located near the ______________ part of the body
d. Joined in the mid-body by the ______________ which extends up to the ______________
e. Has a pair of caudal appendages and two pairs of papillae
a. Measures 1.5 mm x 0.04 mm
b. posterior end: ventrally curved with two lobular caudal appendages
c. it has a single testis located near the posterior part of the body
d. Joined in the mid-body by the genital tube which extends up to the cloaca
e. Cloaca
What is the habitat of Trichinella spiralis?
Small intestine
Skeletal muscle (larva)
Is Man is only an accidental host in Trichinella spiralis?
TRUE
Requires 2 hosts in order to complete the life cycle
Blind Alley Cycle
TRUE OR FALSE:
When humans are infected with T. spiralis it is considered as a dead-end infection
True
LIFE CYCLE OF T. SPIRALIS:
The larvae will lodge itself to the ______ → ______ → ______ → finally lodging itself to the ______
The larvae will lodge itself to the mucosa → lymphatic system
→ circulation → finally lodging itself to the striated muscles
TRUE OR FALSE:
T. spiralis is acquired most commonly to the ingestion of infected pork
True
What Clinical Phase of Trichinella Spiralis is usually seen in the first week of infection
Intestinal Phase
Intestinal Phase:
Small intestinal____ and _______
edema and inflammation
What Clinical Phase can also be known as the incubation/ intestinal invasion?
Intestinal Phase
INTESTINAL PHASE:
a. Signs and symptoms: ______, ______, ______,______, ______, and ______, ______
b. __________ in severe case can also be found as well as ______, and ______,
a. Signs and symptoms: nausea, vomiting, abdominal pain,
diarrhea, headache and fever, myalgia
b. Splenomegaly in severe case can also be found as well as
gastric and intestinal hemorrhages
What Clinical phase of Trichinella spiralis is also known as larval migration and muscle invasion?
Migration Phase
MIGRATION PHASE happens up to the 6th week of infection with the following signs and symptoms:
a. _____________
b. Blurred vision as well as ___________
c. Edema of the ______ and _____
d. _______, _______
e. ___________ (15-40% for 1 month)
f. 4th to 8th week: ________
g. _________ inflammation
h. Edema and pain of _________
i. Larval ________
a. High fever (40oC)
b. ocular disturbances
c. face and eyes
d. Cough, pleural pains
e. Eosinophilia (15-40% for 1 month)
f. 4th to 8th week: death
g. Acute local inflammation
h. Edema and pain of musculature
i. Larval encystation
What are the predominating symptoms of the Migration Phase seen in Trichinella spiralis?
Eosinophilia
Orbital edema
Muscular pain and tenderness
Shallow and painful breathing
General weakness
What Clinical Phase of T. spiralis is where fever, weakness, pain and other symptoms start to abate
Encystment / Encapsulation Phase
Encystment / Encapsulation Phase:
a. Full recovery is expected in this phase since trichinellosis is a
___________
b. (T/F) Self-limiting disease: eventually Trichinella spiralis will die. It just has a specific life cycle once it enters the human body, it
will undergo its life cycle and during this phase the parasite
will die
a. self-limiting disease
b. True
What diagnosis of Trichinella spiralis uses a sample of Gastrocnemius and biceps?
Muscle Biopsy
Muscle biopsy is digested with?
Pepsin-hydrochloride
Muscle Biopsy:
Samples should be taken at week of infection?
3rd or 4th
What diagnosis of Trichinella spiralis uses an antigen preparation of Trichinella larva?
Bachman Intradermal Test
Bachman Intradermal Test observe after how many minutes?
30 mins
Positive result of Bachman Intradermal Test
large elevated swelling at the site of injection
This is done when meat is suspected of harboring the existed larvae of trichinella and feeding the meat to the albino rats
Beck’s Xenodiagnosis
Observation is done 14 days after inoculation for the presence
of female worm in the duodenum, and larvae in the muscles
of the experimental animal.
Beck’s Xenodiagnosis
This would provide the confirmatory diagnosis but the problem with this one is that there is a late rise of the antibody titers which usually commences 3-4 weeks after a light infection
Serodiagnosis
During heavy infections, titers may rise as early as 2 weeks
Serodiagnosis
Enumerate other diagnosis used in the detection of Trichinella spiralis
Serodiagnosis
Beck’s xenodiagnosis
Bentonite Flocculation
Latex Flocculation
IFAT
ELISA
Blood Count: Eosinophilia
Prevention of Trichinella spiralis:
a. Elimination of encysted larva in hogs through _________ at __°C for 24 hours
b. Extermination of _____ and _____ around farms. This would now
prevent the further spread of the parasite
c. Thorough cooking of _____
a. Elimination of encysted larva in hogs through freezing at -30
°C for 24 hours
b. Extermination of rats and mice around farms. This would now
prevent the further spread of the parasite.
c. Thorough cooking of pork
This was described in the Philippines in 1963 after the death of the first human case
Capillaria philippinensis
An epidemic occurred in 1967- 1968 where more than 1000
cases were reported and also 100 individuals died
Capillaria philippinensis
Descendant of Trichuridae
Capillaria philippinensis
Capillaria philippensis:
a. It is a tiny nematode that resides in small intestine of humans
and the esophagus has rolls of secretory cells called
__________
b. The entire esophageal structure is called _________
a. stichocytes
b. stichosomes
Common name of Capillaria philippensis
Pudoc worm
It has a common name of pudoc worm
Capillaria philippensis
What are the diseases related to Capillaria philippensis?
o Capillariasis or Mystery Disease
o Malabsorption Syndrome - Flattening of the villi
Capillaria philippinensis: MALE
a. size: ____ to ____ mm
b. caudal alae; _______, ___________
c. The spicule is ____ - _____ mL
a. size: 2.3-3.17 mm
b. caudal alae; long, non-spiny sheath
c. The spicule is 230- 300 mL
Capillaria philippinensis: FEMALE
a. size: ___ to ___ mm
b. What are 2 Divisions in Females?
o Anterior- __________ and __________
o Posterior- ________ and ______________
c. The vulva is located at the junction of the ________ and the ________ of the body
a. size: 2.4-4.3 mm
b. What are 2 Divisions: Anterior & Posterior
o Anterior- esophagus and esophageal glands
o Posterior- Intestine and reproductive organs
c. The vulva is located at the junction of the anterior and the
middle third of the body
Capillaria philippinensis: FEMALE
a. What are the 2 types of adult female?
a. Atypical and Typical Female