Lesson 10: Hematology

Question 1

Scientific study of blood and its components

Answer 1

Hematology

Question 2

3 Types of Phlebotomy

Answer 2

• Skin Puncture = Capillaries
• Venipuncture = Veins
• Arterial Puncture = Arteries

Question 3

Steps For Phlebotomy

Answer 3

1.) Withdraw blood and place in tube
2.) Centrifuge the blood sample
3.) Formed Elements
- Plasma
- Buffy Coat
- Erythrocytes

Question 4

It transports RBC, WBC and platelets through the blood vessel. It also remove waste products of metabolism

Answer 4

Plasma

Question 5

55% of a normal human’s blood colume

Answer 5

Plasma

Question 6

Anucleated biconcave cells produced from the bone marrow. It transports oxygen. (lungs -> tissue)

Answer 6

Red Blood Cell (Erythrocyte)

Question 7

Gas transporting protein molecule

Answer 7

Hemoglobin

Question 8

Conditions For RBC:
- Low number of RBC
- High number of RBC

Answer 8

• Anemia
• Polycythemia

Question 9

Nucleated cells that lacks hemoglobin. Acts as defense against infection.

Answer 9

White Blood Cells (Leukocyte)

Question 10

Increased Number of Leukocyte

Answer 10

Leukocytosis

Question 11

Decreased number of Leukocyte

Answer 11

Leukopenia

Question 12

Type of White Blood Cell:

• most numerus
• multi-lobed nucleus
• Pale lilac granules
• Function: Immune defense

Answer 12

Neutrophil

Question 13

Type of White Blood Cell:

• Spherical nucleus
• “robin’s egg blue” cytoplasm

Answer 13

Lymphocyte

Question 14

Types of Lymphocyte

Answer 14

1.) T-cell - cellular immune response
2.) B-cell - antibody production
3.) NK cells - kills cancer cells

Question 15

Type of White Blood Cell:
- Usually with bilobed nucleus
- Granules stain bright reddish-orange

Answer 15

Eosinophil

Question 16

Function of Eosinophil

Answer 16

• Defense against parasite
• Activate Allergic response

Question 17

Type of White Blood Cell:

Function:
- Inflammatory response
- Involved in allergic response

Answer 17

Basophil

Question 18

Type of White Blood Cell:
-kidney shaped or horse shoe shaped nucleus

Answer 18

Monocyte

Question 19

Types of Monocyte:

Answer 19

1.) Dendrite Cell: marks out cells that are antigens

2.) Macrophages: act as antigen-presenting cells

Question 20

• cell fragments
• Function: form clots during injury to prevent blood from leaking out

Answer 20

Platelets (Thrombocytes)

Question 21

Increased number of platelets

Answer 21

Thrombocytosis

Question 22

Decrease number of platelets

Answer 22

Thrombocytopenia

Question 23

Complete Blood Count

Answer 23

• hemoglobin
• hematocrit
• red blood cell count
• White blood cell count
• Platelet count/estimate
• rbc indices (MCV, MCH, MCHC)

Question 24

iron-containing oxygen transport metalloprotein in the red blood cells

Answer 24

Hemoglobin

Question 25

3 Types of Method for Hemoglobin Determination

Answer 25

1.) Cyanmethemoglobin Method
2.) Automated Hemoglobinometry
3.) Point-of-care (POC) Hemoglobin Assay

Question 26

What is the reagent used under the cyanmethemoglobin method

Answer 26

Drabkin’s Reagent

Question 27

Principle of cyanmethemoglobin

Answer 27

oxidation of ferrous iron to ferric by potassium ferricyanide = methemoglobin

Question 28

What method is used to utilize cyanmethemoglobin method with modified Drabkin’s reagent

Answer 28

Automated Hemoglobinometry

Question 29

Reagent use for the POC hemoglobin Assay

Answer 29

• Sodium deoxycholate
• Sodium nitrite
• sodium azide

Question 30

It is also known as packed cell volume or erythrocyte volume fraction

Answer 30

Hematocrit Determination

Question 31

Methods for Hematocrit Determination

Answer 31

a.) Spun microhematocrit
b.) Automated

Question 32

Which method is used for manual procedure, its blood collection is skin puncture and uses a microhematocrit centrifuge.

Answer 32

Spun microhematocrit

Question 33

Which method is computed from the mean cell volume and the red cell count

Answer 33

Automated

Question 34

Electrical Impedance is also known as

Answer 34

Coulter Principle

Question 35

Its a Hydrodynamic focusing method

Answer 35

optical detection