Lesson 1 Flashcards
“Micro”
“Bios”
“Logia”
Means?
Small
Life
Study
It is the scientific study of microorganisms, those being of unicellular, multicellular, or acellular.
Microbiology
Types of Prokaryotes
Bacteria
Archaea
It lives in extremely alkaline/acidic environment
Archaea
Types of eukaryotes
Plant cells
Animal cells
A cell wall is present in most but not in all cells?
Prokaryotic cell
It has A cell wall that is present only in plants and fungal cells only
Eukaryotic cell
It has plasma membrane
Eukaryotic and prokaryotic cell
This cell doesn’t have a nucleus
Prokaryotic cell
This cell has a circular or linear, double stranded DNA; genes are not interrupted by introns
Prokaryotic cell
This cell has a linear, double stranded DNA; genes frequently interrupted by intron sequences, especially in higher eukaryotes.
Eukaryotic cells
In prokaryotic cells, cellular organelles are absent except?
Ribosomes
Many of these kinds of cells are strict anaerobes
Prokaryotic cell
A cell with the size of 0.1-10 micron
Prokaryotic cell
A cell with the size of 10-100 micron
Eukaryotic cell
It has both RNA & DNA.
Its nucleus is prokaryotic
It replicates through binary fission
Some of it have motility
bacteria
Nucleus: none
Nucleic acid: either RNA & DNA
Replication: no binary fission
Motility: none
viruses
Nucleus: eukaryotic
Nucleic acid: both RNA & DNA
Replication: binary fission
Motility: none
Fungi
Nucleus: eukaryotic
Nucleic acid: both RNA & DNA
Replication: binary fission & budding
Motility: most
Parasites
It means staff or cane
bakteria
It is the first discovered bacterial shape
Rod shape or bacilli
Singular form of bacteria?
bacterium
What is the single-celled organism that contains both RNA & DNA and reproduce through binary fission?
bacteria
Size of the most pathogenic bacteria is?
1.0-10 micron
They cause most of human infections
bacteria
Steps in binary fission
- Parent cell
- Replication of DNA
- Segregation of DNA
- Cell splitting into two
It is the binomial system in naming bacteria where:
Genera + Specific name = specie
Bacterial nomenclature
He invented the compounding of lenses or compound microscope
Zacharias Janssen
He modified the basic design of the microscope
Robert Hooke
He is the first to discover the cell
Robert Hooke
He described the forms of fungi such as bluish mold on a piece of leather
Robert Hooke
He is the father of microbiology
Antony van Leeuwenhoek
He is first to observe and experiment with microbes
Antony van Leeuwenhoek
This kind of bacteria naturally lives inside the human body w/o causing disease
Normal flora
It is a gram + cocci bacteria that is found in skin or nasal passages. Too much of it may cause boils, impetigo, cellulitis, pimples, and abscess
Staphylococcus aureus
These normal flora maintain skin pH balance and support the immune system of the body
Staphylococcus epidermidis
Propionibacterium acnes
Malassezia fungi
He invented the process that bears his name, pasteurization
Louis Pasteur
He discovered the principle of immunization
Louis Pasteur
These are gram + cocci bacteria that are found in the mouth, throat, skin, gastrointestinal tract.
Streptococcus species
These are the largest gram - bacteria found in the intestines. It helps in digestion and produces vitamins. It can cause a UTI when it enters the urinary tract.
Escherichia coli
He is known for the isolation of microorganisms causing anthrax and tuberculosis
Robert Koch
He developed solid media for culturing bacteria and various techniques to isolate bacteria
Robert Koch
It controls thermoregulation and is triggered by chemicals to kill bacteria or microorganisms
hypothalamus
It is the response of the immune system to any microorganisms, toxins, chemicals
Systematic inflammatory response syndrome (SIRS)
SIRS + microorganisms = ___________ which triggers hypothalamus and causes septic shock
sepsis
Its symptoms are tachycardia, fever, tachypnea
Systematic Inflammatory Response Syndrome (SIRS)
He is the father of antisepsis and developed antiseptic surgical procedures
Joseph Lister
He is the 1st person to isolate the bacteria “Bacillus lactis” in pure form in a liquid culture form
Joseph Lister
He developed the most important staining method in 1884 to visualize bacteria known as “Gram Staining”
Hans Christian Gram
In 1928, he discovered the antibiotic “Penicillin” through studying staphylococcus bacteria where his petri dish was accidentally contaminated by “Penicillium notatum”
Alexander Fleming
He introduced the concept of vaccination
Edward Jenner
These microorganisms are nonpathogenic, their natural habitat is dead organic matter
Saprophytes
Unicellular or metazoan organism living in or on an organism of another species on the expense of the host
parasites
Normal inhabitants of the skin and mucosa, normal flora
Commensals
Classic disease causing microorganisms
Pathogenic microorganisms
Can cause disease in immunocompromised individuals when given a chance
Opportunists or facultatively pathogenic microorganisms
It is the capacity of a pathogen species to cause disease
pathogenicity
It is the sum of the disease-causing properties of a strain of a pathogenic species
Virulence
It is the sum of the disease-causing properties of a strain of a pathogenic species
Virulence
It is the time between infection and manifestation of disease symptoms. It can be measured in hours, weeks, days, months or even years
Incubation period
It is the time between infection and first appearance of products of sexual production of the pathogens
prepatency
It is the totality of host species “susceptible” to infection by a given pathogen
Infection spectrum
It is the smallest number of pathogens sufficient to cause an infection
Minimum infective dose
Method or pathway used by pathogens to invade the host
Mode of infection
Microbiological presence of microorganisms on objects
contamination
Presence of microorganisms on skin without penetration into the tissues
colonization
Invasion of a host by microorganisms
infection
Infection without outbreak of clinical symptoms
Inapparent infection
Infection with outbreak of clinical symptoms
Infectious disease
frequency of clinical manifestation of an infection in disposed individuals
Probability of manifestation
Infection arising from the colonizing flora
Endogenous infection
Infection from the invasion of host by microorganisms from sources external to it
Exogenous infection
Infection acquired during hospitalization
Nosocomial infection
Infection that remains restricted to the portal of entry and surrounding area
Local infection
Lymphogenous or hematogenous spread of invading pathogen starting from the portal of entry
Generalized infection
Systematic disease caused by microorganisms and their toxic products. Pathogens enter the bloodstream, continuously
Sepsis
Brief presence of microorganisms in the bloodstream
Transitory bacteremia
Occurrence of a second infection in the course of a first infection
Superinfection
Series of infection by the same pathogen
Relapses
Series of infection by different pathogens
Reinfection
Factors affecting the growth of bacteria
Air
Temperature
pH
Light
Osmotic pressure
Nutrients
Bacteria that needs oxygen
Aerobic
Bacteria which can grow only in the presence of oxygen
Obligate bacteria
Example of obligate aerobes
Pseudomonas aeruginosa
Bacteria which are ordinary aerobes but can also grow w/o oxygen
Facultative aerobes
Example of facultative aerobes
Escherichia coli
Bacteria that can grow in the presence of low oxygen and in the presence of low (4%) concentration of carbon dioxide
Microaerophilic bacteria
Example of microaerophilic bacteria
Campylobacter jejuni
Campylobacter jejuni which is from raw/half-cooked chicken causes _________
Guillain -Barre syndrome
Bacteria that can grow without oxygen. It lacks superoxide dis mutase and catalase hence oxygen is lethal to these microorganisms
Anaerobic
Bacteria that can grow only in absence of oxygen
Obligate anaerobes
Example of obligate anaerobes
Clostridium species
It causes tetanus, muscle stiffness, and lock jaw
Clostridium tetani
These bacteria require higher amounts of carbon dioxide for their growth
Capnophilic bacteria
Bacteria that can grow well in the presence of 5-10% CO2 and 15% O2
Capnophilic bacteria
Examples of Capnophilic bacteria
Haemophilus influenzae
Brucella abortus
The optimum temp. For most pathogenic bacteria
37 degrees C
These bacteria are cold loving microbes that can grow within a temp. Range of 0-20 degrees. Most soil and water saprophytes belong to this group
Psychrophiles
Example of psychrophiles
Arthrobacter species
Psychrobacter species
These are moderate temp. Loving microbes that grow between 25 and 40 degrees. Most pathogenic bacteria belong to this group
mesophiles
These are heat loving microbes. They can grow at a high temp. range of 55-80 degrees
thermophiles
Example of thermophiles
Bacillus stearothermophilus
Most bacteria grow between pH _______ and __________
7.2 and 7.6
Very few bacteria like ______________, can grow at acidic pH below 4.0
Lactobacilli
Example of bacteria that can grow in an alkaline environment (8.2-8.9 pH)
V. cholerae
Bacteria deriving energy from sunlight
phototrophs
Bacteria deriving energy from chemical sources
chemotrophs
Organisms requiring high osmotic pressures
Osmophilic bacteria
Sudden exposure of bacteria to hypertonic solution may cause osmotic withdrawal of water, leading to osmotic shrinkage of the protoplasm
Plasmolysis
Sudden transfer of bacteria from concentrated solution to distilled water may cause excessive inhibition of water leading to swelling and bursting of cell
Plasmoptysis
Gram positive cocci
Aerobic:
Staphylococcus
Streptococcus
Enterococci
Anaerobic:
Peptostreptococci
Gram positive bacilli
Aerobic:
Bacilli
Cornybacteria
Facultative anaerobes:
Listeria
Anaerobic:
Clostridia
Actinomycetes
Bifidobacteria
Gram negative cocci
Aerobic:
Neisseria gonorrhoeae
Neisseria
Anaerobic:
Veillonella
Gram negative bacilli
Aerobic:
Pseudomonas
Facultative anaerobes:
Salmonella
Escherichia
Proteus
Vibrio
Anaerobic:
Bacteriods
Fusobacterium
Leptotrichia
Gram negative spirochetes
Aerobic:
Leptospira
Anaerobic:
Treponema
Boyrelia
Microaerophiles:
Helicobacter
Spirillum
Innate immunity specific
Intact skin
ciliary clearance
Low stomach pH
lysozyme
Innate immunity non-specific
Eosinophil
Neutrophil
Natural killer
Dendritic cell
Macrophage
Mast cell
Adaptive/acquired immunity active
Natural
vaccination
Adaptive/acquired immunity passive
Maternal
Immunoglobulin treatment
Herd immunity
“chancroid”
Painful genital ulcer
Soft chancre
Haemophilus ducreyi
“Hard chancre”
Sexually transmitted
Genitals, anus, mouth
Treponema pallidum
Flesh eating bacteria
Staphylococcus aureus
Clostridium species
Vibrio vulnificus
Flesh eating bacteria
Staphylococcus aureus
Clostridium species
Vibrio vulnificus