LEPTOSPIROSIS OSPE Flashcards
1
Q
Specimens
A
blood, CSF (first phase), urine (alkaline)
2
Q
Microscopy:
A
Giemsa - spirochaete
Dark ground illumination – actively motile spirochaete
Silver impregnation method – spirochaete
3
Q
Culture:
A
Rabbit Serum Agar
Special media (Korthoff’s, Stuarts, EMJH)
CAM (embryonated eggs)
4
Q
Serology
A
Very diagnostic (Abs detection)
CFT, Agglutination (macro & micro),IFA, SEL, ELISA –
demonstrate Abs
5
Q
OTHER
A
- Animal Inoculation
- PCR
6
Q
Serovar specific test
A
-Microscopic agglutination test (MAT)
-the gold standard
