E.coli OSPE

Question 1

traveler returns from a developing country and complains of severe diarrhea accompanied by nausea and abdominal cramps.
-consuming street food
-Stool samples:Enterotoxigenic E. coli (ETEC).
ELISA toxin assay detects LT or ST toxins.

Answer 1

ETEC

Question 2

10-year-old male with a 4-day history of bloody diarrhea and abdominal pain was admitted.
blood test: low Hb n platelet,high urea,creatinine,LDH
-catalase:+
mac conkey agar: LF
oxidase= -
shiga toxin +

Answer 2

EHEC , O157:H7 serotyping

Question 3

specimen

Answer 3

stool,rectal swab

Question 4

transport medium

Answer 4

cary blair transport medium

Question 5

microscopy

Answer 5

GNB

Question 6

culture

Answer 6

macconkey:pink,LF
Eosin Methylene blue agar:green sheen

Question 7

biochem

Answer 7

catalase:+
oxidase:-
nitrate reduction test:+
tsi:A/A,H2S-.GAS+
IMVIC:++- -

Question 8

serotyping (O,H,Ag)

Answer 8

Agglutination assays
dipsticks
restricition fragment length polymorphism
ELISA

Question 9

MOLECULAR

Answer 9

MJULTIPLEX PCR, DNA PROBES