enteric fever

Question 1

case

Answer 1

fever and fatigue beginning 15 days ago.
* She developed headache, weakness, palpitation, abdominal pain, and diarrhea a week later.
* The patient was apathic, confused and discordant .
* On examination, her fever pattern was rising on a daily basis .
* Her liver was enlarged 5 cm below the costal margin
* Spleen was palpable.

Question 2

sample

Answer 2

blood,serum,stool,urine

Question 3

blood culture

Answer 3

medium:bile broth
method:castaneda’s method

Question 4

State the precaution to be taken in collecting blood for culture

Answer 4

use proper, sterile technique to decontaminate the patient’s skin before collecting the blood culture

Question 5

What are the standard protocol for blood culture in a clinical case suspected with
bacterial infection?

Answer 5

Blood cultures should be drawn prior to initiation of antimicrobial therapy. Preparation of skin prior to blood culture collection is important to prevent contamination of sample. At least two (2) sets of blood cultures should be obtained (each set includes one (1) aerobic and one (1) anaerobic bottle)

Question 6

microscopy

Answer 6

GN BACILLI
MOTILE

Question 7

subCULTURE

Answer 7

blood agar:non-haemolytic colonies
macconkey agar:NLF,COLOURLESS

selective media:SSA,XLD,DCA:BLACK NLF colonies

Question 8

biochem

Answer 8

catalase +
oxidase -
IMVIC: - + - +
TSI:K/A,H2S+

Question 9

BASU

Answer 9

WEEK 1:BLOOD CULTURE
W2:AGGLUTINATION TEST
W3:STOOL CULTURE
W4:URINE CULTURE

Question 10

SEROLOGY

Answer 10

WIDAL TEST:

detect H and O Ag