amoebiasis

Question 1

specimen

Answer 1

fresh dysentric stool,rectal scrapping

Question 2

microscopy

Answer 2

saline mount:motile trophozoite with RBC
Eosin mount: unstained amoebae with pink background
 Iodine mount: for cysts and dead trophozoitesstain glycogen massbrown in
colour
 Iron-haematoxylin stain (permanent stain)stain chromatoid bodiesappear black
in colour
 Trichome stain
if cyst:pathogenic

Question 3

culture

Answer 3

Polyxenic media: Media with enteric bacteria or protozoa (Boeck & Dibohlav’s,
Balamuth’s, Nelson’s media)
 Axenic media; Media without other bacteria or protozoa (Diamond’s medium)

Question 4

Serology

Answer 4

IHA, IFA, Co-agglutination test, ELISA (detects antibody and antigen)

Question 5

Molecular methods:

Answer 5

PCR, DNA probes

Question 6

extraintestinal amoebiasis

Answer 6

aspirate fr liver abcess—–achovy sauce—-trophozoite on microscopy
CT/MRI—other organs