Lectures 17/18: Introduction to Metabolism Flashcards
Cellular metabolism (3)
The chemical processes occurring within a living cell or organism that are necessary for maintaining life
- Provide energy, macromolecules, bioactive molecules from intermittent supply of nutrients
- Prevent build up of toxic materials in wrong place
- Breakdown of xenobiotics
Catabolism
Breakdown of large molecules to release energy and small molecules
Of amino acids, monosaccharides and fatty acids: involves oxidizing carbon
Anabolism
Synthesis of large molecules for storage or biomass using energy
Of amino acids, monosaccharides and fatty acids: involves reducing carbon
Macromolecules (3)
- Energy storage (carbohydrates, fat, proteins)
- Energy transport
- Energy release
Oxidation
Loss of electrons from an atom or molecule
The atom/molecule that loses electrons is being oxidized and is an electron donor
Oxidative, exergonic
Reduction
Gain of electrons by an atom/molecule
The atom/molecule that gains the electron is being reduced and is an electron acceptor
Reductive, endergonic
Oxidation state
Most: CO2, carboxylic acid, aldehyde/ketone, hydroxyl, hydrocarbon: least
Most: triple bond, double bond, single bond: least
Highest redox energy yield
Most reduced to most oxidized: full oxidation to CO2 and H2O
Catabolism of fatty acids provides more energy than catabolism of carbohydrates
Metabolic pathways
Interconverted network of metabolites
Several major metabolic pathways share a few common intermediates
Series of sequential reactions, each catalyzed by a specific enzyme
Redox-active cofactor
When a metabolite is oxidized in catabolic reaction, electron is passed on to cofactor (reduced)
Cofactors can be oxidized again by giving up an electron in anabolic reactions
Many derived from vitamins
Includes NAD/NADH, FAD/FADH2, NADP/NADPH, Q/QH2
Oxidation of cofactors
Occurs during anabolic reaction (NADPH) or during oxidative phosphorylation (NADH, FADH2)
Oxidative phosphorylation
NADH and FADH2 are oxidized, oxygen is reduced to water, and ATP is produced
FAD/FADH2
Cofactor that is usually directly complexed to an enzyme
Ubiquinone (Co-enzyme Q)
Cofactor that accepts two electrons in a stepwise manner to become ubiquinol
Essential
A required nutrient that the human body cannot synthesize de novo
The human body cannot synthesize vitamins
Thermodynamics
Energy changes in metabolic pathways
Directionality
Many pathways are overall reversible, but at any given time, only one direction is active
Flux
Rate of overall pathway
Described how many molecules of substrate are converted to product
Controlled through activity of enzymes catalyzing irreversible reactions
Enthalpy
H
Energy
Reaction is favoured if deltaH is negative
Gibbs Free Energy
A reversible process moves spontaneously in the direction that lowers the systems Gibbs’ Free Energy
Entropy
S
Disorder
Reaction is favoured if delta S is positive
Dynamic equilibrium
Rates of the forward and reverse reactions are the same
Nothing changes in the total amount
The concentrations of products and substrates are not necessarily equal
Equilibrium constant Keq
Defined by the concentrations of the reactants and substrates at equilibrium
Inherent property of reaction
Standard Free Energy Change
DeltaG*’ = -RTlnKeq
Described driving force of equilibrium at standard conditions, when all reactants are present at equal concentrations
Set characteristic of a reaction