Lecture 24: Photosynthesis Flashcards
Photosynthesis
Synthesis of carbohydrates and oxygen in plants
Light dependent and light independent reactions
E + 6CO2 + 6H2O = C6H12O6 + 6O2
Light reactions
Use light energy to oxidize water to oxygen and synthesize NADPH and ATP
Take place in thylakoid membrane: Photosystems 1 and 2
Excitation of reaction centres:
1. Oxidation of water to oxygen
2. Reduction of NADP to NADPH
Generation of transmembrane proton gradient that powers ATP synthesis
Light-independent reactions
Convert CO2 to carbohydrates using NADPH and ATP
Occur within the stroma
Photoreceptors
Variety to light absorbing groups: pigments
Cause different colour of a plant
Chlorophyll, beta-Carotene, Phycocyanin
Absorb light of different wavelengths
Absorbing a photon increases the potential energy of a pigment, energy can be released in different ways when the molecule returns to ground state
Chlorophyll A
Absorbs red and blue light
We see plants as green
Chlorophyll B
Absorbs red and blue light
We see plants as green
Planck’s Law
Energy of light is inversely related to its wavelength
E=hc/wavelength
Light-harvesting complexes
Membrane proteins that contain pigments
Protein environment influences the wavelength of the light that is absorbed
Energy from absorbed photons is transferred between neighbouring chlorophyll to a chlorophyll that acts as a reaction centre
Part of two different super complexes: photosystems 1 and 2
Reaction centres
Primary reactions of photosynthesis occur at specific chlorophyll molecules called reaction centres
Antennae
Other pigments that transfer absorbed light to reaction centre
Photosystem II
Begins light reactions
In chloroplasts grant with little contact with stroma
Contains several light-absorbing pigments and redox-active cofactors as prothetic groups
P680
Chlorophyll dimer Reaction centre of PSII Large absorption peak at wavelength 680 Oxidized form: P680+ Can only be reoxidized after is absorbs light: reduction potential changes dramatically when it absorbs a photon
P680+
Within photosystem II
Strongest biological oxidizing agent known
Captures electrons from water, resulting in O2 and P680
Requires manganese containing cofactor
2H2O = O2 + 4H +4e
P680*
Activated P680 by photon
Very negative reduction potential
Very easily loses an electron and is oxidized
Oxidized by PQ to P680+
Plastoquinone
Accepts two protons and electron from P680* to make plastoquinol
For every H2O, two plastoquinone molecules are reduced
Electrons in plastoquinol are transferred to cytochrome b6f