Lecture Exam 1 Flashcards
Hepatitis C
Hepacivirus (HCV)
Hepatitis B
Orthohepadnavirus
Human Herpesvirus 1
Simplexvirus (HHV-1)
Fever blisters, oral herpes. More common.
Human Herpesvirus 2
Simplexvirus (HHV-2)
Common genital herpes
Human Herpesvirus 3
Varicellovirus
Chicken pox
Flu
Influenzavirus
Aids/HIV
Lentivirus
Measles
Morbillivirus
Gastroenteritis
Norovirus
Papillomavirus
Papillomavirus
Rubella (German Measles)
Rubivirus
Mumps
Rubulavirus
Pertusis (whooping cough)
Bortadella pertussis
Acute gastroenteritis
Campylobacter jejuni
Chlamydia
Chlamydia trachomatis
Colitis
Clostridium difficile
Gangrene, enteritis
Clostridium perfringens
Diptheria
Corynebacterium diphtheriae
Tuberculosis
Mycobacterium tuberculosis
Gonhorrhea
Neisseria gonorrhoeae
Gastroenteritis
Salmonella enterica
Boils, impetigo, sinusitis
Staphylococcus aureus
Pneumonia
Streptococcus pneumoniae
Rheumatic fever, necrotizing fasciitis, strep throat
Streptococcus pyogenes
Candidiasis (oral thrush, vaginitis) (fungi)
Candida albicans
Flu (fungi)
Coccidioides immitis
Pneumonia (fungi)
Pneumocystis jirovecii
Diarrhea (protist)
Cryptosporidium parvum
Giardia
Giardia lamblia (protist)
Malaria
Plasmodium falciparum (protist)
Taeniasis (tapeworms)
Tania solium
How many types of viruses are there?
2, naked and enveloped
What are viruses?
- nonliving entities, not cells
- rely on enzymes/substrates of a host cell
- don’t replicate outside out of a host
- outside a host are completely inert, do not grow or develop
- much smaller than bacteria!
What is a naked virus?
Nucleic acid in a capsid. (Nucleocapsid).
What is an enveloped virus?
Nucleocapsid in a phospholipid envelope. Membrane derived from host membrane or organelles of host membrane.
Glycoprotein spikes
- Function in attachment to host cell
- Found in naked and enveloped virus
- So that virus infects the right cell. Viruses very specific!
What are the 5 types of DNA/RNA that viruses can have, and how quickly can they synthesize proteins?
- dsDNA (immediate)
- ssDNA (immediate)
- dsRNA (needs enzyme)
- +ssRNA (immediate, functions as mRNA)
- -ssRNA (needs enzyme)
What is a bacteriophage?
Viruses that infect bacteria. Found everywhere.
Stages of lytic replication
- Attachment
- Entry - via protein needle
- Synthesis - make RNAs, which are read to make proteins
- Assembly - viruses are assembled
- Release - via lysis of host cell
Plaques on agar due to lytic replication.
What is a temperate phage?
A bacteriophage that can choose between lytic and lysogenic replication?
What is a prophage?
When the temperate phage is within the bacteria’s chromosome. With reproduction both cell DNA and prophage are replicated.
What is induction?
The process by which a dormant prophage detaches from the DNA of a bacterium and switches from lysogenic to lytic replication.
Bacteriophage vs Temperate phage
Bacteriophage is virulent, can infect host cell. Temperate phage injects a dormant prophage, which can use lysogenic or lytic replication.
What is lysogenic conversion?
Presence of a lysogenic phage (or multiple) alters the phenotype of the cell. Viruses do not produce toxins, but cause the bacteria to produce toxins.
What does animal virus replication depend on?
- Virus type
- Type of genetic material
- Same basic steps as bacteriophage
+RNA/-RNA
+RNA contains genetic code
-RNA complement to the code
Attachment in Animal Virus Replication
Glycoproteins/other molecules attach to host cell receptors due to chemical attraction between virus and cell receptors.
Entry of enveloped viruses in Animal Virus Replication
Endocytosis or membrane fusion
Entry of naked viruses in Animal Virus Replication
Endocytosis, or injection of nucleic acid into cell
Steps of Animal Virus Replication
- Attachment
- Entry/penetration
- Uncoating
- Synthesis
- Assembly
- Release
Where does RNA replicate?
Cytoplasm, dsRNA and -ssRNA must carry their own enzymes
Where does DNA replicate?
Nucleus, using host enzymes
First to observe microorganisms
Antonie VanLeeuwenhoek
Used a magnifying glass w/excellent optics, not a compound microscope. Saw “animalcules”
Endosymbiotic theory
Theory that eukaryotic cells developed from prokarkyotic cells
Fluid mosaic model
Structure of a cell membrane is a double layer of phospholipids with protein molecules
Chromosome
Thread-like structure of nucleic acids and proteins that carries genetic information
Organelle
Specialized structure within a cell
Meiosis
Division into 4 daughter cells
Eukaryotic mitochondrion
Ribosome is 70s. Circular DNA. Double membrane.
Cristae - folds created by the inner membrane
Matrix - inside of inner membrane
Chloroplasts
2 membrane, where photosynthesis takes place
Thylakoids
Sites where photosynthesis occurs. Small discs.
Grana
Stacks of thylakoids
Stroma
Surround grana/thylakoids. 70s ribosomes, suggesting that chloroplasts came from bacteria.
Cytoskelteon functions and contents
Maintains cell shape, motility, anchors organelles, vehicle transports, cytokinesis(division)
- Microtubules
- Microfilaments
- Intermediate filaments
Ribosomes
Organelle that synthesizes proteins. Made of rRNA and protein, same function in both bacterial and eukaryotic ribosomes.
Lg - 60s
Sm - 40s
Together - 80s
Golgi body
Packages proteins
Nucleus
2 membrane, 2 phospholipid bilayers. Things go in/out via nuclear pores. Holds DNA, which determines function. Different gene expressions produce different phenotypes
Nucleolus
Site of ribosome biogenesis
Rough ER
protein synthesis
Smooth ER
Calcium storage, lipid synthesis
First to view cells
Robert Hooke, under compound microscope
What are cilia and flagella?
Microtubules. No bacteria have cilia!
Antonie Van Leeuwenhoek
First to observe microorganisms. Used magnifying glass w/excellent optics
Genome
Complete set of genes in an organism
Plasmid
Extra-chromosomal molecule of DNA that replicates independently of the chromosome. Typically circular, typically not in eukaryotes but common in bacteria. Contains non-essential, accessory genes, such as those for antibiotic resistance.
Endoflagellum
Flagella inside the structure of the cell, also called axial filaments.
Taxis
Movement in response to a stimulus (bacterial cells)
Fimbriae vs Pili
Appendages on bacterial cells.
Fimbriae: bristle like proteins used for adhesions. More numerous, often attach to microvilli of intestines.
Pili: long protein appendages, less numerous, used for adherence, some used for motility (reeling in), used for conjugation
Glycocalyx (2 layers)
Layer of complex carbohydrates covering the bacterial cell.
- Capsule: dense, firmly attached, helps escape digestive system by binding to antibodies
- Slime layer (biofilm): loosely attached to the cell, very protective.
Peptidoglycan
Forms bacterial cell wall, consists of amino acids and carbohydrates. Protects from osmotic forces, maintains cell shape.
- Made of NAGs and NAMs
- Thick layer with Gram-positive bacteria
Periplasmic space
The bacterial cell wall is not totally solid, things can flow through the peptidoglycan without transport proteins
Lipopolysaccharide (LPS)
Outer membrane of gram-negative bacteria, comprised of Lipid A, a core polysaccharide, and an O-side chain. (Phospholipids and proteins)
Gram-pos vs Gram-neg membranes
G-pos: plasma membrane, peptidoglycan. Contains teichoic acid/lipoteichoic acid, not found in G-neg
G-neg: plasma membrane, thin peptidoglycan, outer LPS
Acid-fast bacteria
Subgroup of Gram-positive bacteria that has mycelia acid in the cell wall, which is a wax that prevents the gram stain from working properly. Have to use acid-fast stain to visualize. Example: tuberculosis
Flagella purpose
Motility. Rotate from H+ to propel through the environment.
Chemotaxis
Bacterial movement in response to food
Phototaxis
Bacterial movement in response to light