Lecture 8- How are Proteins Studied Outside Cells Flashcards
1
Q
Test of Discovery
A
try to find out the location of our protein of interest
2
Q
Experimental group
A
we do not know what’s going to happen
3
Q
Positive control group
A
event should occur
4
Q
Negative control group
A
even shouldn’t occur
5
Q
Technical controls
A
done to quantify the results of experimental and control groups
6
Q
Purpose of SDS-PAGE
A
to separate proteins by size
7
Q
Steps in SDS-PAGE
A
- coat all the proteins with negative molecules (SDS)
- load gel (hinder their movement)
- Run gel
- soak gel in coomassie blue
8
Q
Coomassie blue staining
A
Purpose: to make all the proteins blue
9
Q
Molecular weight markers
A
tells us the size of our band
also called MW ladders, protein standards
10
Q
Western Blotting
A
shows a specific protein on a gel
11
Q
Detection reagents
A
colorless, but the enzyme can react and turn them into a blue precipitate.
12
Q
Loading control
A
antibodies against a protein that should be present in all samples
