Lecture 8

Question 1

First level of DNA packaging

Answer 1

nucleosome

DNA is copied around 8 histone proteins

Question 2

2nd level of DNA packaging

Answer 2

further compaction or folding of the nucleoside core particles “solenoid structure”

Question 3

histone + DNA

Answer 3

nucleosome

Question 4

protein used to be compacted with DNA

Answer 4

histone

Question 5

transcriptionally active areas associated with relatively open chromatin

Answer 5

euchromatin

Question 6

transcriptionally inactive areas associated with compact or closed chromatin.

has very few genes

Answer 6

heterochromatin

Question 7

long stretches of repeating sequences in heterochromatin

Answer 7

satellite DNA

Question 8

– proposes that posttranslational modifications of histones are read by proteins that bind to modified histones to determine whether regions of the chromosome are transcribed or remain in an inactive state

Answer 8

histone code

Question 9

histones can be either — and —-.

Answer 9

acetylated or methylated

Question 10

associated with open chromatin/unwrapping chromatin

Answer 10

acetylation

Question 11

associated with open or closed chromatin

Answer 11

methylation

Question 12

enzyme used during acetylation

Answer 12

histone acetyl transferase “HAT”

Question 13

enzyme used during deacetlyation

Answer 13

HDAC

Question 14

a subtype of AML. Massive proliferation of myeloid precursors, and the specific chromosomal abnormality has prognostic value and defines treatment.

Answer 14

PML

Question 15

Fuses the PML and RARa genes together
ATRA responsive
Good prognosis

Answer 15

t(15;17)(q25;q21)

Question 16

Fuses the PLZF gene with RARa
Not responsive to ATRA
Not so good prognosis

Answer 16

t(11;17)(q23,q21)