Lecture 7 - Inbreeding, Molecular Evolution and the Neutral Theory Flashcards

1
Q

What are the main importance’s of the bottleneck/founder effect?

A

Reduction in heterozygosity (H) influences fitness immediately after bottleneck
-reduced heterosis, exposure of deleterious alleles
-small initial impact
-influenced by subsequent rate of increase
Reduction in allelic diversity influences ability to respond to long-term selection
-reduced ability to respond to stochastic events
-larger inital impact (on rare alleles)
-less influenced by subsequent rate of increase

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is inbreeding?

A

Mating between two related individuals

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is inbreeding depression?

A

Loss of fitness due to loss of genetic diversity

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What are the features of inbreeding

A
  • inbreeding depression
  • homozygosity of 2 alleles by desend
  • increase in homozygosity in a population
  • genetic subdivision of a population
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Where is inbreeding inevitable and why?

A

Inbreeding is inevitable in small populations
-every individual becomes related quickly becuase it is impossible to find a completely unrelated partner
-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is the formula for the increase in inbreeding in a certain population size?

A

Increase in inbreeding in a random mating population of size Ne is proportional to 1/(2Ne) per generation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

How does genetic drift affect homozygosity?

A

Increased homozygosity through loss of alleles

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

How does inbreeding affect homozygosity?

A

Increased homozygosity due to change in genotype frequencies

-no change in allele frequencies

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What two events are likely in a small population?

A
  • Genetic drift

- Inbreeding

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What two explanations are there for inbreeding depression (loss of fitness due to loss of genetic diversity)?

A

Dominance hypothesis

Overdominance hypothesis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is the dominance hypothesis of inbreeding depression?

A
  • Assumes (partially) recessive deleterious alleles

- Increased number of homozygotes lead to more expressed deleterious alleles

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

What is the Overdominance hypothesis of inbreeding depression?

A
  • Assumes heterozygote advantage/heterosis

- Reduced number of heterozygotes leads to reduced fitness (hybrid vigour)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

How might inbreeding depression change over time under the dominance hypothesis?

A

Purging the genetic load

  • deleterious recessive alleles will be expressed
  • selection can act
  • this leads to allele frequency changes and removes deleterious alleles from the population
  • increased fitness after successive generations of inbred matings
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What evidence is there for purging?

A

Mice
-continued inbreeding in mice shows fitness improvement consistent with the dominance hypothesis
Drosophila
-Base population 1250
-30 lineages derived from base population
-maintained a n=20 for 19 generations
-6 purged populations, each maintained n =250 for 3 generations
-Each of the 6 had 15 full-sibling lines
-inbreeding depression decreased in the purged population compared to the base population

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What are the three formulae involved in loss and fixation due to drift?

A

Frequency of a new mutation: (1/(2Ne))
Chnace of getting fixed = frequency: (1/(2Ne))
Chance of getting lose: 1-(1/(2Ne))

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

What is a mutation (in terms of fixation)?

A

A change that may or may not go to fixation

Mutation rate = μ

17
Q

What is a substitution (in terms of fixation)?

A

A mutation that has gone to fixation and replaced all other alleles at that locus
Substituation rate = k

18
Q

What is the formula for rate of substitution?

A

μ
(Probability of new mutations in a population X probability of fixation of a new mutation)
=> Substituation rate = mutation rate and does not depend on population size

19
Q

What are the features of DNA substitution rates?

A
  • Rates are variable among organisms
  • Rates are variable among chromosomes (and regions within chromosomes) within species (e.g humans and chimpanzees, Y chromosome especially different)
  • rates are variable depending on the type of substitution
20
Q

What are the features of the rate of evolution of the α-Globin gene?

A
  • present in sharks, cats, newt, chicken, echnidna, kangeroo, dog, human etc. (very wide spread)
  • the amino acid substituations over time are a good fit to a straight line
  • this indicates that the rate of α-globin evolution has been relatively constant over the past 450 million years
  • k = 0.9 X 10^-9 amino acid replacements per year
21
Q

What is the molecular clock?

A

When the rate of substitution k = constant

22
Q

What can the molecular clock be used for?

A

-to estimate divergence events, if some divergence events can be dated independently (e.g. fossil record)

23
Q

Give an example of the use of the molecular clock

A

Speciation and the molecular clock on Hawaii

  • Hawaiian islands have been formed in succession
  • Kauai (5.1 Mya), Oahu (3.7Mya), Maui-Nui (1.9/1.6 Mya), Hawaii (0.43 Mya)
  • Mean Cytd distance between the different islands birds’ are a good fit to a straight line against the time since separation
  • Mean Yp1 distance between the different islands drosophila are a good fit to a straight line against the time since separation
24
Q

What are the features of evolutionary rates in different proteins?

A

Data taken from Fibrinopeptides, Hemoglobin, Cytochrome c proteins across species with different divergence times

  • linear lines siggest that rates have not changed over time
  • rates constant among species but differ among proteins
25
Why are the evolutionary rates in different proteins constant among species but differ among proteins?
Selection as the main driving force -more important molecules evolve more rapidly as they are being constantly fine tuned Neutral theory -Less important molecules evolve more rapidly because they have fewer selection constraints
26
What expectations are there from the genetic code about the evolution of sites in chromosomes?
- most synonymous changes are in third position, some in first - more substituations per nucleotide over a long divergence time at synonymous sites - less substitutions per nucleotide over a long divergence time at nonsynonymous sites - sites with fewer selection constraints evolve faster
27
What is the neutral theory?
The neutral theory argues that the most genetic variation at the molecular level is selectively neutral and is therefore maintained by mutation and genetic drift
28
What type of model is the neutral theory and how?
A null model - does not deny that all deleterious alleles are selected against - does not suggest that all morphological, behavioural or physiological differences are the result of drift
29
What is the likely fate of new mutations?
Lost from the population