Lecture 7 - Enzyme Kinetics

Question 1

Substrates bound to enzymes by

Answer 1

Electrostatic bonds
H-bonds
Van der waals forces
hydrophobic interactions

Question 2

Isomerase

Answer 2

catalyze intramolecular rearrangements of molecules

Question 3

reverse reaction of lyase

Answer 3

synthase

Question 4

Molecules that naturally regulate enzyme activity are

Answer 4

reversible noncompetitive inhibitors

Question 5

enzyme is fully active when it has

Answer 5

sugar + ADP

Question 6

Feedback Inhibition

Answer 6

Metabolic pathway turned off by its end product (inhibitor)

Question 7

Kinase adds or removes PO4?

Answer 7

adds

Question 8

transition state complex

Answer 8

increase reaction rates by decreasing the amount of energy required

Question 9

Factors that influence enzyme catalyzed reactions

Answer 9

temperature
pH
enzyme concentration
substrate concentration

Question 10

Km

Answer 10

Substrate concentration at which the reaction rare = ½ maximal value

Question 11

Vmax

Answer 11

Maximal rate of product formation when substrate concentration is high

Question 12

Characterization of Protein

Answer 12

-molecular masss
-isoelectric point
-chromatography

Question 13

Kcat

Answer 13

Measure of catalytic activity