lecture 7 Flashcards
What is the main commonality between bacteria and archae?
both unicellular and both are prokaryotes
What are the main differences between bacteria and archae?
Archae extreme environments
What is the name of the field studying these microbes that can only be individually seen with aid of a microscope?
microbiology
why study microbiology?
medical, food, and industry purposes
Morphological Diversity:
What shapes, arrangements, and motility types exist?
coccus (round)
bacillus (rod)
spirochates/spirillum(spiral)
“strip of strep”->linear
staph->clustered
flagella, axial filaments, gliding (cork screw)
Gram Positive Bacteria and Gram Negative Bacteria and how they differ?
gram negative bacteria is harder to treat, more layers selective porins
gram stain purpose?
distinguishes bacteria
4 main steps of gram stain
- primary stain
- mordant
- 95% alcohol
- counter stain
Bacteria and Archae produce ATP in what 3 ways?
- chemoorganotrophs
- chemolithotrophs
- phototrophs
Bacteria and Archae obtain Building Block carbon in what 2 ways?
- heterotrophs
- autotrophs
Genetic Diversity through Gene Transfer: What are the 3 main methods?
- Transformation- from naked dna
- Transduction- from virus
- Conjugation- from bacteria
Enrichment Definition and example
growing with extra nutrients for robust growth
ex: winogradsky column
Isolation Definition and example
get individual colonies
ex: quadrant streak
3 techniques to further study microbial populations…
how do these 3 differ?
* Metagenomics
* Metatranscriptomics
* Metaproteomics
- Metagenomics- DNA
- Metatranscriptomics- RNA
- Metaproteomics- protein
What does bioremediation mean?
use microbes to clean up pollution
xenobiotics definiton
man made chemicals