lecture 6

Question 1

properties of the bacterial genome

Answer 1

1. single circular chromosome nucleoid region
2. no nuclear membrane (restricted to nucleoid region)
3. plasmids (other small circular self replicating DNA)

Question 2

vertical gene transfer

Answer 2

from parent to offspring (progeny)

Question 3

horizontal gene transfer

Answer 3

directly from one organism to another

pass genes to another already existing bacteria

Question 4

difference between smooth and rough strain

Answer 4

smooth strain has a a polysaccharide capsule

Question 5

3 types of horizontal gene transfer

Answer 5

Transformation
Conjugation
Transduction

Question 6

TRANSFORMATION- e.g Griffith’s experiment

Answer 6

mixed heat killed S strain with live R strain- mouse died
demonstrated horizontal gene transfer
DNA alive moves through cell walls live bacteria

Question 7

TRANFORMATION

Answer 7

uptake of small fragments of naked DNA by naturally transformable bacteria

Question 8

CONJUGATION

Answer 8

transfer of DNA via sexual pilus cell-cell contact

Question 9

TRANSDUCTION

Answer 9

viral vector bacteriophage phage viruses e.g

DNA capsid protein sheath head only attacks very specific bacteriai

Question 10

Lytic and lysogenic cycles exception…..

Answer 10

sometimes instead of repackaging DNA into head, it puts it (plasmid) into another bacteria (horizontal)
adds to genome- extra chromosome