Lecture 5 - Viruses Flashcards
What kind of parasites are viruses?
obligate intracellular
Virion
complete infectious viral particle with nucleic acid surrounded by protein coat
Capsid
protein coat that wraps around the virus
composed of individual capsomeres (protein molecules)
nucleocapsid
RNA/DNA (single or double stranded) + capsid
what are lipid envelopes
layer of plasma membrane from the host that surrounds the nucleocapsid
what kind of viruses have lipid envelopes
influenza, herpes virus, HBV and HIV
what are glycoproteins/spikes?
inserted in the envelopes or capsid –> help the virus attach to the host cell
Two types of protein spikes on influenza virus?
HA (haemagglutinin) [H1] –> attaches to the sialic acid receptors on the red blood cells and cells lining the mucous membranes
NA (neuraminidase) [N1] –> helps virus exit the host cell after replication
Zoonotic infections
infections that can be passed across species i.e. rabies virus (virus of canines that can also infect moose and humans) and West-Nile Virus
What are bacteriophages?
- viruses that infect bacteria
- they can transfer new genes from one bacteria to another
- bacteriophages cannot infect humans
How can bacteriophages be used in scientific labs?
- can be used in genetic engineering
- i.e. producing large amounts of insulin from E.coli
- splice the human gene that makes insulin and then put it into the genome of a bacteriophage (bacteria virus); then have the virus infect e.coli and give it the insulin gene. E.coli will then make mass amounts of insulin for a low cost
What is the negative side effect of bacteriophages?
they can transfer genes across bacteria for production of toxin
i.e. a bacterophage that infects Corynebacterium diphtheriae (causes diptheria) can transfer its virulence factors across multiple bacteria
How do we classify viruses?
grouped into families on the basis of DNA/RNA composition and structure
i.e. we have a group of DNA viruses and a group of RNA viruses
Why are RNA viruses known for their ability to mutate quickly?
Because they require RNA polymerase that the virus brings with itself; this RNA polymerase can convert viral RNA straight into protein (positive) or into mRNA (negative) and by-passes the proof reading ability that DNA polymerase has; therefore it introduces much more mutation than DNA viruses
What are the 6 DNA viruses?
- Papillomaviridae (HPV)
- Adenoviridae (Adenovirus)
- Hepadnaviridae (Hepatitis B virus)
- Herpesviridae (HSV-1, HSV-2, HSV-3…)
- Poxviridae (small pox, monkeypox, chicken pox etc..)
- Parvoviridae (B-19)
How big are viruses compared to bacteria?
viruses are 10-500 nm while bacteria are 500 - 2000nm
What are the three steps that occur in viral infection?
- attachment and penetration into host cell
- Replication of viral genome
- Assembly and Release of New virus
Explain the attachment process of virus to host cell
viruses attach to the host cell usually to their receptors with the help of their glycoproteins/spikes
What are the two processes of penetration of the virus into host cell?
- fusion –> fusion of viral envelope into the host cell and the release of its genome into cytoplasm of host cell
- Pinocytosis –> virus attaches into the receptors and then gets engulfed into the host cell; it then breaks out of its vacoule and releases its genome
How do DNA viruses transcribe in the host cell?
A DNA Virus will hijack the host cell DNA polymerase to produce viral DNA, the viral DNA will then transcribe using the host cell’s RNA polymerase to make mRNA (messener RNA) and leaves the nucleus to the ribosomes to make amino acids to begin protein synthesis