lecture 5 recapn

Question 1

the NF-kB, p53 and HIF pathways all

Answer 1

allow the cell/organism to respond to environmental threats

Question 2

NF-kB is a family of how many proteins

Answer 2

5

Question 3

NF-kB forms dimer complexes which allows them to bind to

Answer 3

DNA

Question 4

rel homology domain is present in all NF-kBs facilitates what

Answer 4

the dimeric complexes forming

Question 5

RelA, RelB and c-rel form a

Answer 5

subfamily

Question 6

transactivation domains allow them to work as what

Answer 6

potent activators of genes

Question 7

p105 and p100 are precursors of which NF-kBs

Answer 7

p50 and p52

Question 8

ubiquitin chains are bound to target protein by

Answer 8

E1, E2 or E3 ligases

Question 9

NF-kB is induced by:

Answer 9

inflammatory cytokines
bacterial products
viral proteins and infection
DNA damage
cell stress

Question 10

NF-kB regulates

Answer 10

immune response
stress responses
cell survival and death
cell proliferation

Question 11

NF-kB dimers are held in an inactive form in the cytoplasm bound to an inhibitory protein called

Answer 11

IkB

Question 12

activation of NF-kB
what is an example of a ligand binds to receptor on cell surface

Answer 12

TFNa

Question 13

binding signals a cascade which results in

Answer 13

activation of the IkB kinase complex

Question 14

what does this complex phosphorylates

Answer 14

IkB

Question 15

what does phosphorylation of IkB signal

Answer 15

ubiquitination of the IkB freeing NF-kB

Question 16

NF-kB goes to the nucleus and does what

Answer 16

binds to target sequences in promotors or enhancers of the genes they regulate

Question 17

IkB kinase complex is made up of what 3 components

Answer 17

NEMO/IKKγ, IKKα or IKKβ

Question 18

the components of the IkB have ______ repeats

Answer 18

ankyrin

Question 19

what do ankyrin repeats do

Answer 19

bind to NF-kB complex and retain it making it inactive

Question 20

what residue on IkB gets phosphorylated

Answer 20

serine residues

Question 21

when NF-kB gets into the nucleus it can be modifies further by

Answer 21

kinases, acetylases, phosphatases and more

Question 22

modifications allows them to selectively interact with other proteins eg

Answer 22

co repressors / activators which could determine whether we get expression or not

Question 23

p300/CBP is a co activator and can interact with and modify

Answer 23

RelA subunit

Question 24

phosphorylation of RelA regulates its ability to interact with p300/CBP as it

Answer 24

opens up its structure and allows binding sites to emerge