lecture 2 recap Flashcards
basal transcription alone results in
low/inactive transcription
UAS acts as a binding site for what
activator proteins which stimulates transcription initiation
what are the common UAS sequences
GC box, octamer and CAAT box
what activator protein binds to GC box
Sp1
what activator protein binds to octamer
Oct-1
what activator protein binds to CAAT box
NFY
where are the UAS sequences located
close to the promoter
response elements are in the
UAS region
they are able to bind to
activator proteins
activator proteins have two domains
DNA binding and activation domain
in prokaryotic activators the binding domain and activation domains are
integrated into one region
in eukaryotic activators they are
separate and linked by a flexible protein domain
examples of DNA binding domains are
leucine zipper, zinc finger, homeodomain and helix loop helix
activation domains interact with
other proteins in the transcriptional machinery e.g TAFs
how do activators work
1. binding of first activator to UAS promotes
binding of a second activator to a different UAS
