Lecture 5 Flashcards
1
Q
Adult Stem Cells
A
- Rare and found in clusters
- Characterised by expression of developmental markers
- Capable of multi-potent differentiation
2
Q
What are Endothelial Progenitor Cells?
A
- cells that facilitate angiogenesis
- VEGF, Angiopoietin-1, GM-CSF = Inc. mobilisation
3
Q
What are the 4 major elements of tissue engineering in vivo?
A
- Cells - differentiated or stem cells
- Scaffold - hydrogel, solid, decell.
- Growth factors - survival, prolif., vasularisation
- Vascularisation
4
Q
Features of Cardiac Tissue Organoids
A
- ring structures engineered with hydrogels
- cells = cardiomyocytes
- scaffold = collagen hydrogel
5
Q
Benefits & Limitations of Hydrogels
A
Benefits:
- Simple & Modifiable scaffold
- Cells controlled vol.
Limitations:
- Avascular
- Vol. limited
- Integration
- Low contractile force
6
Q
Benefits & Limitation of Cell Sheets
A
Benefits:
- Simple
- Modifiable cells
- Integration
- Stacking
Limitations:
- Avascular
- Vol. limited
- Low contractile force
7
Q
Benefits & Limitations of Decelluarised Scaffold
A
Benefits:
- ‘Rapid’ Assembly
- 3D
- Large vol.
- Transplantable
Limitations:
- Complex procedure
- Donor tissue limitations
- Low contractile force
8
Q
Benefits & Limitations of In Vivo Vascularised Chamber
A
Benefits:
- vascularised
- 3D
- large vol.
- modifiable scaffold cells
- transplantable
Limitations:
- complex procedure
- Low contractile force
- Integration
