Lecture 16 Flashcards
1
Q
Characteristics of Regulatory T-cells (Tregs)
A
CD4+ & CD25+ T-cells
–> essential for control of autoreactive T cells in vivo
–> Many Treg phenotypes most co-express CD25+
–> Suppress immune responses via:
o Cell-cell interaction
o Cytokine production
2
Q
What is pMP6a?
A
= Mammalian Expression Vector
3
Q
Characteristics of pMP6a
A
- bacterial origin of replication
- Ampicillin resistance gene
- Cytomegalovirus promoter
- Unique restriction site after promoter
4
Q
What does pMP6a vector with?
A
- mIL-10
- vIL-10
- TGF-b gene inserts
