Lecture 5 Flashcards
which of the following fluids would provide the most accurate results for carbohydrate testing?
a) CSF
b) urine
c) wholeblood
d) all of the above
c) wholeblood
T/F only diabetes mellitus is responsible for glucose changes in the body
F
which of the following is false regarding glucose oxidase (trinder’s)?
a) good to use plasma, CSF, urine and whole blood without issues
b) only reacts with beta D glucose which makes up 64% of glucose
c) must at mutarotase to convert any glucose into beta D glucose
d) bilirubin and bleach can both cause interferences with the reaction
a) good for plasma and CSF because urine and whole blood may have to have interferences removed
name 2 things that may cause interference with glucose oxidase (trinder’s) and what it does to the results?
- bleach would falsely increase
- bilirubin would falsely decrease (interferes with glucose ability to react w/ enzyme)
how do glucose oxidase and glucose dehydrogenase differ?
GO: has more interferences
GD: highly specific and very little interference
may add mutarotase to both to change alpha to beta
which of the following methods do not require mutarotase?
a) glucose oxidase
b) glucose dehydrogenase
c) hexokinase
d) they all require it to change beta-D glucose
c) hexokinase
which of the following is the most accurate in measuring glucose?
a) glucose oxidase
b) glucose dehydrogenase
c) hexokinase
d) they are all equally accurate
c) hexokinase
T/F hexokinase can be used for serum or plasma
true
which of the following may cause interference with hexokinase?
a) hemolysis
b) drugs
c) lipemia
d) icteric samples
e) all of the above
e) all of the above
T/F the dose of glucose is the same for adults, children, and pregnant women
F, varies based on age and pregnant women get less
what is the first step when someone comes in for an oral glucose tolerance test?
take blood before giving them their dose of glucose, then take after
T/F for an OGTT, a patient must discontinue any medications that interfere with glucose metabolism
F, only if it is safe to do so
which of the below is not something that a patient may be asked to do for an OGTT?
a) do not smoke or consume caffeine during test
b) ensure they eat a low carb diet prior to the OGTT
c) avoid strenuous movement and stress
d) not be vissibly ill or bedridden
b) try to have a normal and unrestricted diet leading up to your OGTT
which of the following may contribute or cause lab errors during the OGTT?
a) insufficient or incorrect info from patient
b) incorrect labeling of tubes without time differentiating
c) insufficient patient monitoring
d) incorrect carb load given
e) all of the above can cause errors
e) all of the above
which of the following is a consideration that must be made for glucose measurements for CSF specimens?
a) incubate at room temperature for 20 minutes to increase glucose levels
b) centrifuge and then put in the fridge at 4C
c) put in the freezer at -20 until ready to run
d) it is STAT and must be centrifuged ASAP to remove cellss
d) STAT and must be centrifuged ASAP to remove cells
T/F CSF glucose measurement can be used as a standalone test result
F, must do serum with CSF bc things affect glucose in CSF so you wanna correlate CSF value with serum value
T/F the reference value for CSF glucose is >2.5mmol/L or 2/3 of the serum value
true
whole blood is ___% lower than plasma/serum glucose
a) 10-15%
b) 12-16%
c) 12-15%
d) 11-15%
c) 12-15%
in a separated, non-hemolyzed sample, serum glucose is stable for?
a) 8 hours at 4C and 72 hours at 25C
b) 8 hours at 22C and 72 hours at 4C
c) 8-72 hours at 22C
d) 8 hours at 25C and 72hrs at 4C
d) 8 hours at 25C and 72hrs at 4C
non separated whole blood glucose may decrease by how much an hour?
a) 6%
b) 7%
c) 8%
d) 9%
e) it does not decrease as long as you put it in 4C
b) 7%
glycolysis is inhibited by ______ because it chelates _______
a) sodium fluoride;calcium
b) sodium citrate; magnesium
c) sodium fluoride; magnesium
d) sodium citrate; calcium
c) sodium fluoride; magnesium
which of the following is true regarding capillary and arterial blood specimens?
a) capillary and arterial blood have slightly lower glucose [], more easily affected by recent food, and higher oxygen [] that may affect enzyme tests
b) capillary and arterial blood have slightly higher glucose [], more easily affected by recent food, and higher oxygen [] that may affect enzyme tests
c) capillary and arterial blood have slightly higher glucose [], less easily affected by recent food, and lower oxygen [] that may affect enzyme tests
d) capillary and arterial blood have slightly lower glucose [], less easily affected by recent food, and lower oxygen [] that may affect enzyme tests
b) capillary and arterial blood have slightly higher glucose [], more easily affected by recent food, and higher oxygen [] that may affect enzyme tests
which of the following is true about newborn and infant glucose levels?
a) critical glucose value depends on their age
b) have lower blood sugar
c) may be transient and asymptomatic and may be due to mothers diabetic state
d) all of the above
d) all of the above
which of the following is false regarding HgbA1C?
a) A1C is formed when glucose in blood binds irreversibly & spontaneously to hgbA
b) once the sugar binds to Hgb, it is able to detach if the body needs it
c) not recommended as the sole diagnostic tool, but good results indicate better all around health
d) diabetics will have a higher target % than non diabetics
b) once the sugar binds to Hgb, it is able to detach if the body needs it
once it binds it stays for the entirety of the RBC life!
